The standard final concentration for each dNTP in a PCR reaction is 200 micromolar. Higher concentrations can increase error rates by reducing the fidelity check by the polymerase. They can also chelate magnesium ions, reducing cofactor availability. For long-range PCR amplifying fragments over 10 kb, concentrations of 400 to 500 micromolar per dNTP are sometimes used to sustain extension through long amplicons.
PCR & qPCR
PCR Reagents: dNTPs, Ladders, RNase Inhibitors
PCR accessory reagents: dNTPs, DNA ladders, gel stains and RNase inhibitors
The reagents and consumables around your polymerase matter as much as the enzyme itself. This is true whenever you set up a PCR or qPCR experiment, since even minor lapses in reagent quality can compromise a clean result. These PCR reagents cover the full set you need alongside your master mix.
Individual dNTPs at 100 mM stock and ready-to-use 10 mM dNTP mixes let you use the exact nucleotide concentration your protocol requires. DNA ladders cover 100 bp through multi-kilobase ranges for your standard gel sizing, with colour-coded ladders available for fast band orientation.
Nucleic acid stains include non-toxic ethidium bromide alternatives for agarose gel visualisation, added directly to the gel before casting. RNase inhibitors protect your RNA during cDNA synthesis and RT-qPCR setup, with options for standard research scale through high-throughput workflows.
Molecular-grade and universal agaroses support routine gel electrophoresis. Extraction controls monitor nucleic acid recovery across your full workflow and flag extraction failures before amplification results are read.
Choose the right accessory for each step
1) dNTPs for polymerase reactions. Individual 100 mM dNTPs suit you when your protocol specifies exact concentrations, while a 10 mM dNTP mix covers most standard PCR setups where a balanced nucleotide pool is enough.
2) DNA ladders. A 100 bp ladder suits your products under 1 kb, while a 1 kb or larger ladder covers amplicons above that range. Colour-coded ladders simplify band identification on the gel without needing to count from the well.
3) Gel staining. Non-toxic ethidium bromide alternatives are added directly to your agarose at standard concentrations. They work with standard UV transilluminators and offer a safer alternative to intercalating dye disposal.
4) RNase inhibitors. For RT-qPCR and cDNA synthesis reactions, an RNase inhibitor protects your RNA template before reverse transcription, with options available for standard research volumes and for high-throughput formats.
5) qPCR controls. RT-PCR extraction controls monitor nucleic acid recovery across your entire workflow and flag extraction failures before amplification. They are available in multiple fluorescent formats for multiplexed monitoring.
Applications
Custom PCR reaction assembly
Individual dNTPs set precise nucleotide concentrations rather than a fixed mix, useful for protocols needing an exact ratio, paired with PCR master mixes.
Carryover prevention and labelling
Individual dNTPs support low-dUTP UNG carry-over prevention in your diagnostic workflows, plus nick-translation labelling needing a specific analogue.
Gel sizing and fragment verification
DNA ladders guide fragment-size assignment during electrophoresis, supporting PCR verification, restriction mapping, and library size checks.
Non-toxic gel staining
Non-toxic stains replace ethidium bromide in routine agarose gel imaging, working with standard UV transilluminators without added intercalating-dye disposal burden.
RNA protection for cDNA synthesis
RNase inhibitors protect your RNA templates during synthesis, mattering most for low-input samples and long-read sequencing prep, alongside RT-qPCR kits.
Extraction quality control
Extraction controls flag failed recovery across clinical, food safety, and environmental PCR testing, confirming a true negative result, alongside purification kits.
Frequently asked questions
Protein-based RNase inhibitors bind and block the active site of secreted RNases, particularly RNase A, RNase B, and RNase C. They are used during cDNA synthesis reactions to prevent RNA template degradation before or during reverse transcription. They matter most when working with low-abundance RNA, or clinical or tissue-derived samples that may carry trace RNase contamination. They also matter in any workflow where the RNA must stay intact throughout multiple sequential enzymatic steps.
A 100 bp DNA ladder contains bands spaced 100 bp apart in the 100 to 1000 bp range. It gives precise size resolution for small PCR amplicons, restriction digest fragments, and short oligonucleotides. A 1 kb ladder provides bands at 1 kb intervals or in a logarithmic series. This makes it suited to larger PCR products, genomic restriction fragments, and plasmid linearisation checks above 1 kb. Using the wrong ladder compresses band spacing and makes accurate size assignment difficult.
Product catalogs
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