Standard PCR detects amplification endpoint by gel electrophoresis and is qualitative. Quantitative PCR (qPCR) measures fluorescence each cycle, allowing calculation of a Ct value that correlates with starting template quantity. qPCR is therefore used when copy number, transcript level, or pathogen load must be measured numerically.
Products by Technology
PCR Master Mix, qPCR Kits, Polymerases, and Reagents for PCR Workflows
PCR master mixes and qPCR kits for reliable amplification and quantitative results
This range spans the full PCR and qPCR workflow, from enzyme and buffer selection through custom oligonucleotide synthesis to supporting reagents. Each sub-category targets a specific method, so you can match components from your DNA template to Ct value at every step.
PCR Enzymes and Master Mixes covers the full range, from low-cost Taq mixes for colony screening to proofreading high fidelity polymerases for your cloning and long-range amplification. Formats include standard 2x mixes, hot-start variants, direct amplification mixes that bypass DNA extraction, and isothermal master mixes for loop-mediated isothermal amplification (LAMP/RT-LAMP). Isothermal amplification options include Air-Dryable and Lyo-ready RT-LAMP master mixes. These give you an ambient-temperature stable, field-ready alternative to your standard thermal cycling setup.
qPCR Kits provides SYBR Green and probe-based master mixes for quantitative PCR, matched to your instrument platform. RT-PCR and RT-qPCR covers one-step kits that run reverse transcription and amplification together for your samples, two-step cDNA synthesis kits, and miRNA-specific quantification systems. Inhibitor-tolerant qPCR and RT-qPCR master mixes are also available. They support direct amplification from your crude or complex sample types.
Pathogen Detection by PCR offers validated TaqMan assay kits for bacterial, viral, fungal, and plant pathogens, including lyophilised field formats and research-grade (some products may be available in diagnostic-grade formats; confirm intended use and regulatory status for the specific product and jurisdiction) variants. PCR Accessories and Reagents supplies dNTPs, DNA ladders, nucleic acid stains, RNase inhibitors, agarose, and extraction controls to support and validate your workflows. qPCR Probe and Primer Synthesis provides custom hydrolysis probe and primer synthesis with Black Hole Quencher chemistry for probe-based quantification.
Find the right product category for your experiment
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Routine PCR amplification. Go to PCR Enzymes and Master Mixes for standard Taq mixes, hot-start enzymes, and high fidelity polymerases.
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Real-time quantification from DNA. Go to qPCR Kits for SYBR Green or probe-based master mixes matched to your instrument's ROX requirement.
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Gene expression or miRNA quantification from RNA. Go to RT-PCR and RT-qPCR for one-step kits, cDNA synthesis kits, and miRNA quantification systems.
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Pathogen identification. Go to Pathogen Detection by PCR for target-specific validated TaqMan kits covering bacterial, viral, and plant pathogens.
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Supporting reagents. Go to PCR Accessories and Reagents for dNTPs, ladders, gel stains, RNase inhibitors, and extraction controls.
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Custom probes and primers. Go to qPCR Probe and Primer Synthesis for dual-labelled hydrolysis probes and matched primer sets.
Applications
Gene expression profiling
RT-qPCR quantifies mRNA abundance across your treatment conditions, time points and tissue types. See RT-PCR and RT-qPCR kits for transcript-level resolution.
Pathogen detection
PCR detects pathogens in your clinical, food, and environmental samples. See TaqMan kits, when appropriately validated; always confirm regulatory status for your jurisdiction.
Genotyping and SNP discrimination
Hydrolysis probes in a qPCR format distinguish single-nucleotide polymorphisms, supporting your research genotyping and applied genomics work.
Gene knockdown and editing validation
RT-qPCR confirms siRNA silencing efficiency and verifies transcriptional outcomes after your CRISPR editing, giving a quantitative readout.
Product Categories
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PCR Accessories & Reagents
Loading dyes, enhancers, nucleotides, and plates for setting up, optimising, and running PCR experiments.
View ProductsPCR Enzymes & Master Mixes
High-fidelity and routine polymerases and ready-to-use master mixes for standard and challenging PCR amplification.
View ProductsRT-PCR & RT-qPCR
Reverse transcriptase enzymes and one-step RT-qPCR kits for sensitive gene expression analysis from RNA templates.
View ProductsqPCR Kits
SYBR Green and probe-based qPCR kits with optimised buffers for accurate, reproducible quantification of nucleic acid targets.
View ProductsFrequently asked questions
The cycle threshold (Ct), also called Cq, is the cycle at which fluorescence crosses a defined threshold above background. Lower Ct values indicate more starting template. A difference of 3.32 cycles between two samples corresponds to a ten-fold difference in starting quantity, assuming 100 percent amplification efficiency.
Probe-based assays use a sequence-specific hydrolysis probe that fluoresces only when the correct amplicon is present, giving higher specificity. SYBR Green intercalates into any double-stranded DNA, which can include primer-dimers. Probes are preferred for multiplexing, low-abundance targets, and pathogen detection where off-target signal must be excluded.
Commonly used reference genes include ACTB (beta-actin), GAPDH, HPRT1, B2M, and RPLP0. No single reference gene is universally stable across all experimental conditions. Best practice is to validate two or more candidate reference genes in your specific cell type, tissue, and treatment context using a stability algorithm such as geNorm or NormFinder before applying them to experimental data.
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