PCR & qPCR
These validated PCR kits include a matched positive control and established sensitivity data for most targets. Kits come in two formats. One is a TaqMan probe and primer set bundled with a matched positive control and master mix. The other is a probe and primer set only, for use with your own master mix, ready to run on standard real-time PCR instruments. The PCR and qPCR kits are compatible with most commercially available real-time PCR detection systems, so you can fit them into your existing setup.
Targets include food-safety pathogens such as Listeria monocytogenes, Salmonella, and E. coli O157. They also include regulated plant pathogens such as Xylella fastidiosa and Candidatus Liberibacter solanacearum. Other targets include respiratory viruses such as SARS-CoV-2, influenza A and B, and veterinary targets such as mastitis-causing bacteria.
Many assays come in standard and lyophilised formats. Lyophilised kits stay stable at ambient temperature. This suits field-based or point-of-care screening. Where available, CE-marked in vitro diagnostic (IVD) products carry the appropriate regulatory clearance for their intended use. Diagnostic-grade variants are available for selected targets. Each kit includes a TaqMan extraction control to confirm RNA or DNA recovery from your sample. This lets you flag failed extractions before you interpret amplification results.
1) Food pathogen screening. A standard TaqMan PCR kit works well with your extraction protocol.
2) Plant pathogen surveillance. The lyophilised format suits field sampling where you cannot guarantee cold-chain continuity, staying stable at ambient temperature for transport and storage.
3) Respiratory virus detection. Target-specific TaqMan RT-PCR kits cover SARS-CoV-2. Combined multiplex kits detect influenza A, influenza B, and SARS-CoV-2 at the same time in a single tube.
4) Probe and primer only. If you use your own master mix, a probe and primer set without the master mix component suits you. This also suits multiplexing into an existing detection channel.
5) Extraction controls. An RT-PCR extraction control in your workflow monitors nucleic acid recovery and flags extraction failures before amplification.
Food safety testing
Quantitative TaqMan assays detect and count bacterial pathogens such as Salmonella, Listeria monocytogenes, and Campylobacter, in enrichment cultures or raw samples.
Plant pathology and biosecurity surveillance
Real-time PCR identifies regulated plant fungi, bacteria, and viruses. Lyophilised formats suit field screening without refrigeration.
Respiratory virus diagnostics
Multiplex RT-qPCR panels detect influenza A, influenza B, and SARS-CoV-2 at the same time in a single tube, cutting your turnaround time for panel testing.
Veterinary diagnostics
Target-specific TaqMan kits detect mastitis-causing bacteria and other veterinary pathogens, supporting herd health monitoring alongside food-safety testing.
Extraction and workflow quality control
Extraction controls built on nucleic acid purification chemistry run alongside every sample, flagging failed extractions before you report results.
Custom and multiplexed research workflows
Probe and primer-only formats, built from custom qPCR probe synthesis, support your bespoke panels for microbiology and environmental research.
PCR detects pathogen-specific DNA or RNA sequences with very high analytical sensitivity. It often reaches one to ten genome copies per reaction. Unlike culture, PCR does not need viable organisms and gives results within hours rather than days.
However, PCR detects nucleic acid from both live and dead cells. Combining PCR with a short enrichment step or propidium monoazide pre-treatment can help you distinguish viable from non-viable cells in food or environmental applications.
An extraction control is a defined nucleic acid spike added to the sample before extraction begins. It passes through every processing step alongside the target. After amplification, the extraction control signal shows whether extraction, purification, and amplification worked correctly.
A missing extraction control signal points to an extraction failure or the presence of PCR inhibitors. In that case, repeat the test rather than report a false-negative result.
Common inhibitors include polysaccharides and polyphenols in plant matrices, and haeme compounds in blood and meat. Humic acids in soil and environmental water, and calcium ions in dairy products, are also common. Inhibitors reduce amplification efficiency or can suppress the PCR signal completely.
You can manage inhibitors in several ways. Dilute the extract, or add a wash step to the extraction protocol. You can also use inhibitor-tolerant master mix formulations, or monitor every sample with an extraction or internal amplification control.
Yes. Multiplex PCR uses several primer-probe sets in one tube. Each probe carries a different fluorophore, so you distinguish targets by detection channel. Multiplexing cuts cost and processing time when you screen panels of closely related pathogens or respiratory viruses.
Assay design needs a balance of primer Tm values and concentrations, so all targets amplify with similar efficiency. Include a no-template control for each fluorescent channel.
Product catalogs
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