RNase Inhibitors

Recombinant RNase inhibitors for RNA protection during isolation, RT, and qPCR

Protein-based RNase inhibitors protect RNA from degradation during isolation, in vitro transcription, RT, and qPCR. Standard inhibitors are recombinant placental RNase inhibitors with broad inhibition spectrum across mammalian RNase A-family enzymes. Specialised variants offer thermostability or relaxed-buffer compatibility.

Pick by thermostability and buffer compatibility.

1. Routine RT and qPCR. Standard recombinant placental RNase inhibitor works at standard reaction temperatures.
2. One-step RT-qPCR at elevated annealing temperatures. Choose thermostable inhibitor; standard inhibitor activity collapses above 50C.
3. Concentration. Match to your reaction volume; over-supplementing wastes inhibitor without benefit.
4. Buffer compatibility. Some buffer compositions reduce inhibitor activity. Check compatibility with your downstream chemistry.
5. Storage. Verify storage stability; freeze-thaw cycles reduce activity.

Meridian Life Science and Lucigen supply the standard catalogue.