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Protein Arrays and Antibody Arrays for Multiplex Detection

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Antibody and protein arrays for multiplex analyte detection in one experiment

These platforms measure dozens of analytes at once, including cytokines, chemokines, growth factors, soluble receptors, and many more. Sample volume needs vary by format, matched to your available material.

Many biological processes depend on multiplex signalling networks. Apoptosis, inflammation, angiogenesis, immune responses, and cell migration all involve extensive crosstalk between pathways. To understand these processes, you need multiplex data rather than single-analyte readouts in your model system.

The portfolio covers focused application categories, including Angiogenesis Arrays, Apoptosis Arrays, Autophagy Arrays, Bone Metabolism Arrays, Chemokine Arrays, Disease Focused Arrays, DNA Damage Arrays, ECM Arrays, Growth Factor Arrays, Inflammation Arrays, Isotyping Arrays, Neuroscience Arrays, Pathway Arrays, Stem Cell Arrays, and T Cell Arrays, for your research area. It also spans several platform types.

  • Proteome Profiling Arrays. Glass slide microarrays that measure thousands of proteins at once, in a small sample volume.
  • Cytometric Bead Arrays (CBA). RayPlex® bead-based multiplex assays run on your standard flow cytometer, using RayBiotech's validated antibody pairs.
  • Phosphorylation Arrays. These track how your cells regulate key processes, including the cell cycle, cell growth, and apoptosis.
  • Protein Arrays. These screen protein-protein and protein-ligand interactions, profile autoantibodies, and identify receptors in your samples.
  • Peptide Arrays. Peptide epitope mapping arrays for antibody epitope mapping and detection of IgG, IgA, IgM, and IgE.
  • PTM Arrays. Post-translational modification (PTM) arrays for multiplex detection of acetylation, acylation, nitration, nitrosylation, oxidation, and SUMOylation.
  • Glycobiology Arrays. These profile glycosylation, giving you insight into disease processes.

The range spans focused panels of around 40 markers up to several thousand proteins on a single slide.

Choosing the right array platform for your panel

  1. Array type / platform. The options span membrane-based (semi-quantitative) arrays, glass-slide semi-quantitative arrays, Quantibody / multiplex-ELISA quantitative arrays, cytometric bead arrays (CBA), and label-based high-density arrays. Your choice depends on whether you prefer a chemiluminescent, fluorescent, ELISA-style, or flow cytometry readout.

  2. Analytes. The biomarkers that must be included come first, for example inflammatory cytokines, chemokines, growth factors, autoantibodies, or pathway markers, and the panel that best covers your target list follows from there. Broader discovery arrays or custom designs work where a standard panel does not fit.

  3. Species. The array needs validation for your species of interest, for example human, mouse, or rat. Cross-reactivity should not be assumed unless it is explicitly documented.

  4. Sample type. The array should match your sample, such as serum or plasma, cell culture supernatant, cell or tissue lysate, or other body fluids. The chosen product needs validation for your sample type and its recommended input amount.

Applications

Cytokine and chemokine discovery

Membrane and label-based arrays screen dozens to thousands of secreted proteins in your cell lysates, conditioned media, and serum in one experiment.

Cancer biomarker screening and pathway mapping

Membrane and label-based arrays map pathway activation and screen your candidate biomarkers in cell lysate and serum, supporting oncology research.

Inflammatory disease and immuno-oncology profiling

Glass-slide arrays measure relative cytokine signal across hundreds of targets on one slide, useful in your inflammatory disease research.

Immune cell subset and CAR-T monitoring

Cytometric bead arrays profile T-helper subset cytokines on your standard flow cytometer, useful for CAR-T and checkpoint-inhibitor research.

Therapeutic antibody and autoimmune epitope mapping

Peptide mapping arrays characterise therapeutic antibody epitopes and map autoantibody targets relevant to your autoimmune disease research.

Autoantibody repertoire profiling

Protein microarrays profile serum autoantibody repertoires across cohort studies, supporting biomarker discovery in translational research.

Frequently asked questions

What is the main difference between membrane-based and glass-slide antibody arrays?

Membrane-based arrays use chemiluminescence detection on standard western blot imaging equipment. They give semi-quantitative spot densities relative to a positive control. Glass-slide arrays use fluorescence scanning to give semi-quantitative relative signal across hundreds to thousands of targets on one slide. For absolute concentration values in pg/ml, choose a quantitative platform such as Quantibody or multiplex ELISA. Glass-slide arrays offer higher target density, while membrane arrays offer simpler instrumentation requirements.

How do antibody arrays compare to mass spectrometry for protein profiling?

Antibody arrays deliver targeted, quantitative results for defined panels of analytes within hours, using standard laboratory equipment. Mass spectrometry provides unbiased discovery across thousands of proteins, but it requires specialised instruments, complex sample preparation, and bioinformatics pipelines. Arrays suit hypothesis-driven studies or validation of a known target list. Mass spectrometry is preferred for untargeted discovery.

What sample types are compatible with antibody arrays?

Most antibody arrays accept serum, plasma, cell culture supernatant, and cell or tissue lysate. The accepted sample type and volume depend on the specific array format. Glass-slide and membrane arrays typically need 50 to 200 microlitres of biological fluid, or a defined amount of total protein from lysate. Check the array's protocol for matrix-specific dilution recommendations.

Can I use an antibody array to identify new biomarkers?

Yes. Broad-coverage label-based arrays that screen 1,000 or more proteins are designed for discovery-phase experiments comparing disease versus control samples. You can then validate differentially expressed proteins identified by the array individually, using ELISA or western blot. This array-then-ELISA workflow is a standard approach in translational biomarker research.

Product catalogs

Not sure which catalog to start with? We will help you find the right products.

Tell us what you are looking for — antibodies, kits, proteins, or supplies — and our team will point you to the best catalog filters, suppliers, and product matches for your workflow.

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