A label-based array prints capture antibodies against hundreds to thousands of target proteins onto a glass slide or membrane at defined positions. Your sample proteins are labelled directly with biotin before the assay. You then incubate the biotinylated sample with the array, where each target binds its matching antibody spot. A streptavidin-HRP or streptavidin-fluor conjugate binds the biotin label, and the signal at each spot gives a relative readout of protein abundance.
Antibody & Protein Arrays
High-Density Antibody Arrays for Broad Proteome Screening
High-density antibody arrays for broad protein and cytokine profiling
Screening hundreds of proteins usually means running many separate assays, one target at a time. High-density antibody arrays map protein and cytokine profiles across a broad panel from a single sample, so you skip running individual ELISAs for each target.
Label-based arrays work by labelling your sample proteins directly with biotin. You then incubate the biotinylated sample with the printed antibody array, where each target binds its matching capture antibody. A streptavidin-HRP or streptavidin-fluor conjugate reveals the biotin label at each spot, giving you a relative, semi-quantitative readout of protein abundance.
Panels range from focused sets up to broad, high-density arrays that screen up to around 8,000 proteins in a single assay. Coverage includes cytokines, growth factors, receptors, adipokines, proteases, signalling proteins, metabolic enzymes, structural proteins, epigenetic markers and neuroregulatory factors relevant to your research.
Arrays come on glass slide or membrane formats, so you can match the array to the equipment you already have.
Choosing a High-Density Array Format
- If you need the broadest protein coverage in a single assay: high-density panels screen up to around 8,000 targets, giving you the widest simultaneous readout for discovery-phase screening.
- If you want strong coverage of cytokines, growth factors and receptors: the same high-density panels give you extensive lists across these classes, alongside adipokines, proteases and signalling proteins.
- If sample volume is limited: label-based arrays need as little as 20 to 30 microlitres of serum or plasma per array.
- If you prefer not to rely on a dedicated scanner: membrane-format arrays read out without extra imaging equipment. Glass slide arrays need a compatible laser scanner, or a slide-scanning service if you do not have one.
- If you need defined concentration values rather than relative signal: label-based arrays are semi-quantitative. For absolute values, a quantitative format such as Quantibody arrays is the better fit.
Applications
Expression and phosphorylation profiling
High-throughput profiling maps changes across a broad panel from one sample, replacing separate assays for each target.
Drug target discovery
Broad protein panels help you flag potential molecular targets for drug development, narrowing a wide field of candidates before deeper validation by ELISA.
Mechanism of drug action
Comparing protein profiles before and after treatment helps uncover the molecular mechanisms behind how a drug works, across many targets in parallel.
Disease process research
Screening a broad target panel helps identify factors involved in disease processes, giving you a wide starting point for follow-up study.
Biomarker discovery
Biomarker panels screen for markers linked to disease management, ahead of confirming leading candidates with quantification kits or a targeted assay.
Disease classification
Expression and phosphorylation patterns from one array support molecular classification of disease subtypes. For defined values, quantitative arrays fit better.
Product Catalog
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Label-based High-Density Arrays
High-Density Antibody Arrays for Broad Protein and Cytokine Profiling
Frequently asked questions
High-density arrays screen the broadest set of targets, up to around 8,000 proteins, for open-ended discovery work. Targeted cytokine arrays cover a focused panel of immune mediators chosen for a specific context, such as inflammation or T-cell biology. Use a targeted array when you already have a defined hypothesis. Use a high-density array when the relevant pathway or target is not yet known.
You measure spot intensities from the chemiluminescent or fluorescent image, usually by densitometry. Most array kits include analysis software or a template that maps intensities to the antibody layout and normalises to the positive control spots on the array. Fold-change comparisons between conditions are the standard output, since results are relative rather than absolute concentrations.
The array typically includes internal positive controls for normalisation and blank negative controls to set background. Biological replicates across conditions help you distinguish real expression differences from sample-to-sample variation. Running a reference sample on multiple arrays lets you normalise between them.
Parent category: Protein arrays
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