Label-based High-Density Arrays

High-density antibody arrays for broad protein and cytokine profiling

Screening hundreds of proteins usually means running many separate assays, one target at a time. High-density antibody arrays map protein and cytokine profiles across a broad panel from a single sample, so you skip running individual ELISAs for each target. Label-based arrays work by labelling your sample proteins directly with biotin, which binds its matching capture antibody on the printed array before a streptavidin-HRP or streptavidin-fluor conjugate reveals a relative, semi-quantitative readout at each spot. Panels range from focused sets up to broad, high-density arrays screening around 2,000 proteins in a single assay, covering cytokines, growth factors, receptors, adipokines, proteases, signalling proteins, metabolic enzymes, structural proteins, epigenetic markers and neuroregulatory factors.

Pick by panel size and readout.

1. Panel size. 1000+ analyte broad survey arrays versus disease-focused panels of 50 to 200.
2. Species. Human, mouse, rat coverage varies.
3. Readout. Chemiluminescence versus fluorescence. Scanner versus film.
4. Sample. Verify your matrix is supported (serum, plasma, lysate, urine, conditioned media); volumes range 30 microliters to 1 mL.
5. Quantification. Semi-quantitative reference-based versus fully quantitative with standard curves. Use semi-quantitative for discovery; confirm hits by ELISA or Luminex before drawing conclusions.

RayBiotech leads with the C-Series, G-Series, and Quantibody arrays. Full Moon BioSystems specialises in phosphorylation-pathway arrays.