It is a multiplex sandwich immunoassay printed on a glass slide. Capture antibodies for many analytes are spotted in replicate, and each is detected with a matched antibody. A standard curve converts signal to absolute concentration, so you measure many proteins at once with ELISA-like precision.
Antibody & Protein Arrays
Quantitative Antibody Arrays
Quantitative antibody arrays for absolute protein concentrations across multiplex panels
This format combines the specificity of a sandwich immunoassay with the multiplexing power of an array, so you can measure many targets at once from a single small sample.
Capture antibodies are spotted in replicate on a glass slide. Each analyte is detected with a matched biotinylated detection antibody, followed by a streptavidin-conjugated fluorophore. A standard curve, run alongside your samples, converts signal to concentration.
The result is absolute quantification of tens to hundreds of proteins from a single small sample, with ELISA-like precision. This requires calibration with appropriate standards within the validated dynamic range.
Panels are organised by disease area or analyte class. This lets you profile a cytokine response, an angiogenesis pathway, or a custom marker set in one experiment.
Every analyte is quantified against its own standard. This means results compare directly across your samples, time points and studies. A fluorescence slide scanner reads the array, and analysis software fits the curves and reports concentrations.
Panel and quantification need guide the choice.
1) Absolute concentrations across many analytes. A quantitative antibody array suits real protein concentrations, not relative intensity, for tens to hundreds of your targets at once.
2) Panel selection. A disease-focused or analyte-class panel that matches your biology covers most needs. A custom panel built from validated antibody pairs suits your target list when nothing standard fits.
3) Sample conservation. This format suits limited sample volume. Multiplexing measures many analytes from a small aliquot that would otherwise run only one or two of your singleplex ELISAs.
4) Cross-study comparison. Standard-curve quantification suits work where your results must compare directly across batches, time points or sites.
5) Readout. A fluorescence slide scanner is used to read the array. If you do not have access to a compatible scanner, a slide-scanning and data-extraction service covers your readout.
6) When semi-quantitative is enough. If you only need to see which analytes change, a membrane-based array read by chemiluminescence may be the simpler choice.
Applications
Cytokine and chemokine profiling
Inflammatory, infection, and immuno-oncology markers come together as a coordinated panel, with absolute concentrations reported for every analyte in your set.
Biomarker discovery and validation
Patient cohorts screened for candidate biomarkers give validated hits with absolute concentrations that compare directly across your studies and cohorts.
Pathway analysis
Angiogenesis, apoptosis, or growth-factor signalling pathway assays come together as a coordinated panel, rather than one analyte at a time.
Translational and preclinical studies
Multiplexing measures many analytes from one small aliquot, conserving your sample compared with running separate singleplex ELISA kits.
Secreted-protein profiling in cell and tissue models
Secreted-protein output from your cultured cells or tissue explants is tracked with a standard curve for each analyte in the panel.
Custom biomarker panels
A custom panel built from validated antibody pairs suits your defined target list, as an alternative to a fixed disease panel or bead-based multiplex assay.
Product Catalog
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Quantibody Arrays (Quantitative)
Quantitative Antibody Arrays for Multiplex Protein Concentration Measurement
Frequently asked questions
A membrane array gives semi-quantitative, relative signal. It is read by chemiluminescence without special equipment. A glass-slide quantitative array gives absolute concentrations from a standard curve and is read on a fluorescence scanner.
Multiplexing measures many analytes from one small aliquot. This means a quantitative array conserves sample compared with running separate singleplex ELISAs. Typically only about 50 ul of sample is needed to quantify up to 40 proteins in quadruplicate, which makes the assay markedly more sample-efficient than running individual ELISAs. The exact volume depends on the panel and the number of replicates.
Yes. Alongside fixed disease and pathway panels, custom panels can be assembled from validated antibody pairs. This lets you quantify a defined marker list in a single multiplex run.
Product catalogs
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