RayPlex Multiplex Bead Antibody Arrays

Quantitative Multiplex Bead Immunoassays

• More data with less sample
• 4-hour processing time
• 5-10x more cost-effective than ELISA
• Compatible with most flow cytometers
• No specialized or dedicated instrument(s) needed
• Customized arrays for Human, Mouse and Rat
• Premade arrays

RayPlex Bead Arrays couple the versatility of RayBiotech´s vast antibody pair library with familiar, reliable flow cytometry methodology. Together this creates an affordable, customizable, high throughput multiplex bead-based array requiring no dedicated instrument. RayPlex is compatible with most standard flow cytometers. RayBiotech´s trusted expertise in antibody array design delivers a bead array with unparalleled reliability and sensitivity.

For a customized assay please choose your validated antibody pairs for your Cytometric Bead-based Array from over 800 target proteins to assemble a customized array for human, mouse, or rat.

Research Applications

• High-throughput profiling of cytokine expression
• Validation of semi-quantitative antibody array results
• Identifying potential molecular targets for drug development
• Identifying the molecular mechanisms of drug action
• Identifying crucial factors involved in disease processes
• Discovering biomarkers for disease management
• Discovering expression patterns for molecular classification of diseases

How it Works

RayPlex arrays are first prepared by immobilizing capture antibodies onto small beads of different sizes and different fluorochromes; there is only one capture antibody per bead size-fluorochrome combination. The capture antibodies bind to their specific protein targets during sample incubation, and unbound proteins are removed with washing. Biotinylated detection antibodies and PE-conjugated streptavidin molecules are added, thus enabling protein detection via the PEstreptavidin-biotin-antibody complex. Individual proteins are identified by their specific bead-fluorochrome combination, while the level of PE fluorescence reflects the amount of protein that has been captured to the beads. The protein amount can be determined (i.e., quantified) by comparing the PE signal to a standard curve generated from purified protein standards at known concentrations.

A schematic showing how RayPlex enables multiplex protein detection with flow cytometry.

RayPlex MagPro Bead Arrays

RayPlex®-MagPro kits use magnetic beads. The kits provide the ability to magnetically purify the beads rather than vacuum or centrifugal separations. The magnetic microspheres allow for an improved sorting option while opening the door to automated bead sorting. Unlike plastic beads, which require centrifugation or filtration for sample washing and separation, magnetic microspheres can be quickly collected with a magnetic separator.