Glass-slide antibody arrays are semi-quantitative. The fluorescence signal at each spot reflects the relative amount of that target in your sample, not an absolute concentration from a per-analyte standard curve. This lets you rank targets and compare samples reliably. If you need absolute concentration values in pg/ml, choose a quantitative platform, such as Quantibody or multiplex ELISA, instead.
Antibody & Protein Arrays
Glass Slide Antibody Arrays for Semi-Quantitative Multiplex Protein Profiling
Glass slide antibody arrays for high-sensitivity, multiplex protein profiling from small samples
RayBio G-Series arrays print sandwich antibody pairs onto a glass slide, the same size as a standard histology slide, and read out via fluorescence rather than chemiluminescence. This gives a wider dynamic range than membrane-based formats, letting you screen dozens to hundreds of proteins from your sample at once.
A biotinylated detection antibody cocktail binds your captured analytes, followed by a streptavidin-conjugated fluorophore. You then scan the slide with a laser-based microarray scanner. If you do not have access to a scanner, RayBiotech offers a free slide-scanning and data-extraction service for all glass-slide arrays.
The glass substrate gives high sensitivity and a wide dynamic range: detection limits reach the low pg/ml to ng/ml range, across around four orders of magnitude. The readout is semi-quantitative. It reports a relative fluorescence signal for each target, not an absolute concentration calculated from a per-analyte standard curve. Available panels cover human, mouse, and rat cytokines, chemokines, growth factors, and soluble receptors for your species.
Array sizes range from focused panels of around 40 targets to larger multi-analyte configurations. Only 10 microlitres of your original sample is needed per array, which suits studies with limited biological material and large sample numbers. A single technician can process dozens of arrays a day.
Incubation chambers and frames from the array accessories range are made specifically for glass-slide formats. They control your sample volume and prevent cross-contamination between wells, and work across our semi-quantitative and label-based glass-slide array formats.
When to Use Glass Slide Arrays vs. Other Formats
- If you want a sensitive, semi-quantitative view of many proteins at once: glass-slide arrays are the fit. Fluorescence detection gives a wider dynamic range and higher sensitivity than membrane chemiluminescence formats, with a relative signal reported for each target. If you need absolute concentration values in pg/ml, a quantitative platform fits better instead, such as Quantibody or multiplex ELISA.
- If you are comparing several samples side by side under identical conditions: many G-Series formats print multiple identical sub-arrays on one glass slide, so you can run several samples in the same assay and remove inter-assay variation.
- If you have very limited sample material: glass-slide arrays need as little as 10 microlitres of your original sample per array, which suits studies with restricted biological material or large sample numbers.
- If you do not have access to a fluorescence scanner: RayBiotech's free slide-scanning and data-extraction service is available, or the membrane-based format works instead, using chemiluminescence on standard western blot imaging systems.
- If you need to process many samples in a high-throughput setting: glass-slide arrays suit automated processing. A single technician can process dozens of arrays a day, and the format is compatible with many automated array processing systems.
Applications
Inflammation and cytokine profiling
10 to 40 cytokines and chemokines screen at once from your serum or bronchoalveolar lavage, using as little as 10 microlitres per array.
Immuno-oncology and tumour microenvironment mapping
Cytokine and checkpoint-protein levels map across your paired pre- and post-treatment samples, with hits confirmed by ELISA.
Secreted protein comparisons in cell biology
Secreted protein profiles compare across your conditions on one slide, removing inter-assay variation. Array accessories keep volumes consistent.
Biomarker discovery and candidate shortlisting
Relative expression ranks across a broad panel to shortlist candidates from your limited sample volume, with hits confirmed on Quantibody arrays.
Cross-species translational studies
Cytokine, chemokine, and growth factor profiles compare across matched human, mouse, and rat panels to bridge your animal-model and human sample data.
Autoimmune and rheumatology profiling
Synovial fluid screens for cytokine and chemokine signatures to characterise inflammatory joint disease activity in your model and track changes over time.
Product Catalog
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Glass-based Arrays (G-Series)
Glass Slide Arrays for High-Sensitivity Multiplex Protein Profiling
Frequently asked questions
Glass-slide antibody arrays need as little as 10 microlitres of original sample per array, for serum, plasma, cell culture supernatant, or cell lysate. This small volume is diluted before loading, so the exact dilution and working volume depend on the specific array's protocol. Check the kit insert for exact requirements.
Glass-slide arrays use fluorescence detection to give a semi-quantitative relative signal for each target, with higher sensitivity and a wider dynamic range than membrane formats. Membrane arrays use chemiluminescence on standard western blot imaging equipment and give semi-quantitative spot densities, normalised to positive controls. Glass-slide arrays need a fluorescence scanner, or you can use the manufacturer's free slide-scanning service. Membrane arrays work on imaging equipment most labs already have.
Glass-slide arrays need a laser-based microarray fluorescence scanner that can read the excitation and emission wavelengths of the streptavidin-conjugated fluor used in the kit. If you do not have access to a suitable scanner, RayBiotech offers a free slide-scanning and data-extraction service for all glass-slide arrays. Check the array protocol for the exact wavelength requirements before you choose a scanner.
Parent category: Protein arrays
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