Exosome Isolation Kits and Extracellular Vesicle Tools

Exosome isolation kits and extracellular vesicle tools for every workflow stage

The right isolation chemistry for your downstream assay carries through labelling, characterisation, and functional studies without switching supplier. Extracellular vesicles (EVs) include small EVs in the sub-200 nm range, commonly called exosomes. They carry protein, RNA, and lipid cargo that reflects their cell of origin. Size alone does not establish endosomal biogenesis. Preparations are best described as small EVs or exosome-enriched fractions in your results, until characterised by orthogonal methods.

Three established isolation routes are available for this workflow, alongside the downstream tools needed to work with the recovered EVs. Polymer precipitation is covered by SBI System Biosciences ExoQuick and ExoQuick-TC, for your plasma, serum, and conditioned media. ExoQuick ULTRA variants suit applications requiring lower protein carryover in your samples.

Size-exclusion chromatography is covered by SBI System Biosciences SmartSEC EV isolation systems (Single, Mini, and HT formats for serum and plasma). These use a proprietary contaminant-trapping resin for higher purity and EV concentration in your prep than conventional SEC. Silicon Carbide (SiC) column purification is available through the Norgen Biotek exosome and total RNA range. It suits nucleic acid recovery from your EV-containing samples, and RNA-focused workflows where protein contamination would interfere.

Downstream tools cover the rest of the workflow. These include EV RNA and DNA extraction, fluorescent labelling, and tetraspanin antibodies for CD9, CD63, and CD81. Magnetic bead capture runs through the SBI Exo-Flow IP kits, alongside ELISA-based EV quantification. An EV engineering platform supports cargo loading, surface display, and bioluminescent reporters. FBS exosome depletion kits and the ready-to-use Exo-FBS media supplement prepare EV-depleted culture media for your cell culture work.

Products cover serum, plasma, urine, CSF, saliva, and conditioned media starting materials, whatever your sample type. For service-based characterisation, including nanoparticle tracking analysis and LC-MS/MS proteomics, exosome analysis services cover sample submission through to project quotation. A wider sample-to-insight overview lives at exosomes.biocat.com.

Matching an isolation method to your sample and readout

1. Starting sample and downstream readout. For plasma, serum, or conditioned media with a functional or marker readout, use ExoQuick polymer precipitation. For plasma or serum where intact EV quality and reduced co-isolated contaminants matter for your study, use SmartSEC size-exclusion chromatography with contaminant trapping. For RNA recovery from your EV-containing samples, use Norgen SiC column purification.

2. Throughput. For your single sample or low-throughput research, use SmartSEC Single or Mini, or ExoQuick. For higher throughput serum and plasma processing, use SmartSEC HT. For RNA workflows scaled to your study size, use Norgen exosomal RNA columns.

3. Capture by marker. The question may be which CD9, CD63, or CD81 positive subpopulation carries your cargo. Exo-Flow magnetic bead IP kits handle the tetraspanin pull-down.

4. Cell culture preparation. Background EVs need removing from FBS before any conditioned-media experiment in your lab. FBS exosome depletion kits or the ready-to-use Exo-FBS Media Supplement handle it for you.