Exosome Isolation Reagents for Serum, Plasma, Urine and Conditioned Media

Exosome isolation kits for serum, plasma, urine and conditioned media, without ultracentrifugation

The method you choose here sets your purity, throughput, and downstream assay in one workflow step. The BioCat portfolio covers three technology routes. Polymer precipitation uses the ExoQuick family. Size-exclusion chromatography (SEC) with proprietary contaminant trapping uses different SmartSEC variants. Silicon carbide (SiC) column purification uses Norgen Biotek kits.

ExoQuick and ExoQuick-TC are the core polymer-precipitation products, for plasma or serum and for ascites or tissue culture media. ExoQuick UltraPure variants add a built-in column clean-up step for your workflow. This cuts albumin and immunoglobulin carryover. It makes them the preferred choice for your proteomics and other work that needs lower protein contamination.

SmartSEC is a chromatographic size-exclusion method with proprietary contaminant trapping. It keeps the strengths of SEC, including separation by particle size, high purity, reproducible results, and intact EVs. Pores in the bead core carry affinity groups that capture small protein impurities, such as albumin and IgG, up to around 400 kDa, while the inert outer shell lowers non-specific binding. EVs elute in a single, concentrated fraction, ready for your downstream assay.

SmartSEC comes in three formats, so you can match the format to your sample volume and throughput. Single handles 100 to 250 ul, Mini works from as little as 10 ul for precious samples, and the 96-well HT plate processes up to 96 serum or plasma samples in under an hour. All formats give higher purity and yield than ultracentrifugation, ready for your mass spectrometry, NTA, TEM, Western blot, and RNA-Seq work.

SmartSEC-DeLipo adds lipoprotein removal for blood-derived samples, where lipoproteins such as ApoB and ApoE carry their own RNA and can mask true EV signals. It pairs SmartSEC separation with an affinity DeLipo step, available as a standalone column or with SmartSEC, in single-column and 96-well HT formats. It is validated for plasma, serum, and breast milk, giving you lipoprotein-depleted EVs for your RNA, proteomics, and functional assays.

Norgen SiC column kits purify intact EVs from a wide range of biofluids using silicon carbide resin. The purified exosomes are free of RNA-binding protein contamination. This makes SiC the method of choice when exosomal RNA integrity is your priority.

Select your exosome purification kit by sample type and purity requirement.

1. Sample type. For your serum or plasma: use ExoQuick or ExoQuick UltraPure, SmartSEC for clean SEC isolation, or SmartSEC-DeLipo when lipoproteins must be removed. Conditioned media: use ExoQuick-TC or an ExoQuick UltraPure variant for your culture. Urine, CSF, or saliva: use Norgen SiC column kits or ExoQuick for your low-volume biofluids.

2. Purity requirement. For the highest purity, and for proteomics, lipidomics, or mass spectrometry, use SmartSEC for SEC with contaminant trapping, SmartSEC-DeLipo for blood samples that need lipoprotein removal, or ExoQuick UltraPure. For standard purity, and for miRNA profiling, ELISA, or qPCR, use ExoQuick or ExoQuick-TC. When RNA integrity is your priority, use Norgen SiC column kits.

3. Throughput. For single or low-n samples, use SmartSEC Single or Mini, ExoQuick individual tubes, or Norgen columns. For your high-throughput cohorts, use the 96-well SmartSEC HT plate, or SmartSEC-DeLipo HT when lipoprotein removal is needed. For large-volume conditioned media, scale up with ExoQuick-TC formats to fit your volume.

4. Lipoprotein removal. For your plasma or serum where lipoprotein contamination would confound results, use SmartSEC-DeLipo. For standard precipitation without lipoprotein depletion, use ExoQuick or ExoQuick-TC.