The ExoMS Total Protein Capture Kit lyses EVs and captures every protein inside and on the vesicle, using an affinity purification strategy. The ExoMS Surface Protein Capture Kits use a two-step biotinylation and bead capture process instead. This isolates only the proteins exposed on the outer EV membrane, without lysing the vesicle. Both approaches reduce common carryover contaminants, such as albumin and IgG, for improved LC-MS/MS detection of low-abundance biomarkers.
Extracellular Vesicles / Exosomes
Exosome Proteomics Sample Preparation Kits for LC-MS/MS Analysis
Exosome protein capture kits for fast, low-carryover LC-MS/MS sample preparation
ExoMS Protein Capture Kits prepare your extracellular vesicle (EV) proteins for LC-MS/MS proteomics. They remove common co-isolated proteins such as albumin and IgG, so your mass spectrometer can detect more low-abundance biomarkers that traditional methods often miss.
Three kits cover the sample types you work with. The ExoMS Total Protein Capture Kit uses affinity-based purification of pre-isolated EV preparations. This gives broad total EV proteome profiling, including intraluminal and membrane-associated proteins. The ExoMS Surface Protein Capture Kit (Serum/Plasma) and the ExoMS Surface Protein Capture Kit (Tissue Culture) use a two-step biotinylation and bead capture method instead. Each isolates only the surface and membrane proteins, the EV "surfeome", enriching the biomarker candidates you need that whole-lysate methods often miss.
All three kits accept EVs already isolated by precipitation reagents, ultracentrifugation, or most column-based kits. Sample preparation takes under five hours, from your isolated EVs to a protein preparation ready for LC-MS/MS.
For broader EV characterisation alongside your proteomics work, exosome analysis services support the characterisation of extracellular vesicles from your samples.
Choose your ExoMS kit by proteome target and sample source
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Proteome target. For the full EV proteome you need, including intraluminal proteins, use the ExoMS Total Protein Capture Kit. For surface-exposed proteins only, such as receptors, tetraspanins, and ligands, use an ExoMS Surface Protein Capture Kit.
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Sample source. For EVs isolated from serum or plasma, use the ExoMS Surface Protein Capture Kit (Serum/Plasma). For EVs isolated from tissue culture media or other biofluids, use the ExoMS Surface Protein Capture Kit (Tissue Culture). The ExoMS Total Protein Capture Kit works with EVs from your biofluid of choice.
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Compatibility. All three kits accept EVs that you have already isolated by precipitation reagents, ultracentrifugation, or most column-based kits sold by other vendors.
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Required EV input. All kits need pre-isolated EVs as starting material. Input recommendations vary by kit format and your reaction size.
Applications
Biomarker discovery
Compare total EV protein cargo between disease-state and healthy-donor preparations by LC-MS/MS. Confirm candidates with your targeted exosome quantification assays.
Surface proteome profiling
Surface Protein Capture Kits enrich receptors, tetraspanins and ligands for target discovery. Profile hits with exosome marker antibodies.
EV engineering quality control
Confirm that surface proteins display as expected on engineered EV batches. Pair this check with exosome engineering tools used for cargo loading.
Low-abundance marker detection
Reduced albumin and IgG carryover improves detection of low-abundance surface markers relevant to your drug-target discovery and biomarker validation studies.
Product Catalog
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Exosome Mass Spectrometry Sample Prep
Fast, Low-Carryover Protein Capture for Exosome LC-MS/MS Prep
Frequently asked questions
Use the ExoMS Surface Protein Capture Kit (Serum/Plasma) if you want surface proteins only, or the ExoMS Total Protein Capture Kit if you need the full EV proteome. The ExoMS Surface Protein Capture Kit (Tissue Culture) is formulated for EVs isolated from tissue culture media or other biofluids, not serum or plasma.
All three ExoMS kits work with EVs already isolated by precipitation reagents such as ExoQuick, ultracentrifugation, or most column-based kits sold by other vendors. Kits require pre-isolated EVs as starting material; contact BioCat for guidance on isolation method and input amount.
Each ExoMS kit takes isolated EV samples to a protein preparation ready for LC-MS/MS in under five hours. This is faster than many traditional in-solution digestion workflows built around separate lysis and clean-up steps.
Normalise input by total protein content or particle number before sample preparation. Process all samples in the same batch where possible, to keep run-to-run variability low. Label-free quantification (LFQ) and tandem mass tag (TMT) multiplexing are common quantification strategies for ExoMS-prepared samples.
Product catalogs
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Tell us what you are looking for — antibodies, kits, proteins, or supplies — and our team will point you to the best catalog filters, suppliers, and product matches for your workflow.
