Store at minus 80 degrees C in single-use aliquots. Avoid repeated freeze-thaw cycles. Each cycle causes membrane vesicle fusion and particle aggregation, which you can detect by NTA as a shift toward a larger mean diameter. Adding trehalose (50 to 100 mM) as a cryoprotectant reduces aggregation during freezing. For short-term use within one week, storage at 4 degrees C is acceptable.
Extracellular Vesicles / Exosomes
Purified Exosomes Ready-to-Use from Human Cell Lines
Purified exosomes ready to use from human cell lines for consistent EV studies
These preparations skip the in-house isolation step, taking you straight to defined, characterised EV material. Each preparation gives you a consistent reference point for positive controls, uptake studies, and functional assays across experiments and laboratories. EV preparations from defined human cell lines provide material with confirmed size distribution, and for many preparations, tetraspanin surface marker expression data that supports consistent comparison between laboratories.
Cell lines used for production include cancer-derived lines and standard expression cell lines. Where characterisation data are provided, lots are assessed by nanoparticle tracking analysis (NTA), confirming a mean particle diameter in the 100 to 200 nm range. Where indicated on the Certificate of Analysis, lots are also assessed by Western blot for tetraspanin markers CD63, CD81, and/or CD9. Not all preparations carry every validation assay. The Certificate of Analysis lists the specific characterisation data available for your lot. Cells are cultured in exosome-depleted media before EV harvest, to minimise bovine EV contamination. Preparations are supplied frozen and should be aliquoted on receipt.
Choose your purified exosome preparation by cell source and intended application.
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Cell source for biology. Cancer-derived EVs suit your tumour biology uptake studies or cargo analysis from disease-relevant cell lines. HEK293 EVs are the standard production cell line for your cargo-loading experiments and as a reference preparation in EV engineering work.
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Application type. For a positive control for your ELISA or Western blot, any cell line preparation works, and widely used cell lines give the broadest community reference. For uptake and biodistribution imaging, a fluorescent EV labelling kit applied after receipt suits your tracking. For functional cargo delivery studies, lentiviral cargo-loading systems paired with the preparation support active loading before use.
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Particle number. The Certificate of Analysis confirms particle concentration before use, and diluting to your assay working concentration with PBS or an appropriate buffer follows from that figure.
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Characterisation data. The Certificate of Analysis covers the characterisation data for your specific lot. Many preparations include NTA size and concentration data, plus Western blot confirmation of one or more tetraspanin markers relevant to your application. The specific assays vary by preparation.
Applications
Oncology and tumour biology
Cancer cell line derived EVs model tumour-to-stroma communication, immune-checkpoint ligand transfer, and metastatic niche formation for your oncology research.
Drug delivery and engineered vesicle benchmarking
Defined EV preparations with known particle counts and protein cargo give your engineered vesicles a reproducible performance benchmark.
Assay positive controls
Cell line EV preparations with Certificate-of-Analysis data serve as positive controls, confirming EV quantification assay sensitivity before your clinical samples.
Uptake and biodistribution imaging
A fluorescent EV labelling kit applied to purified exosomes after receipt tracks cellular uptake or tissue distribution in your model.
Functional cargo delivery studies
Purified exosomes paired with lentiviral cargo-loading systems support active loading before use in your functional delivery experiments.
EV engineering reference material
HEK293-derived EVs are the standard production cell line for cargo-loading experiments and serve as a defined reference preparation in your EV engineering work.
Product Catalog
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Purified Exosomes
Ready-to-Use Purified Exosomes for Consistent EV Studies
Frequently asked questions
Typical uptake assay doses range from 10^8 to 10^11 particles per ml of recipient-cell media, depending on cell type and assay sensitivity. Start with a dose-response experiment across two to three log orders, to identify the linear uptake range before you commit to a fixed dose. The NTA-confirmed particle concentration on the Certificate of Analysis is the most reliable basis for dose calculation.
Check the Certificate of Analysis for the characterisation data provided with your lot. This may include NTA size distribution (expected peak 80 to 150 nm) and Western blot confirmation of one or more tetraspanin markers. If you plan to use the preparation in functional assays, a tetraspanin ELISA on an aliquot gives an independent quantitative check.
Cell-line EVs give you reproducible, high-yield reference material. However, they differ from primary-cell or biofluid EVs in cargo composition and surface protein expression, reflecting the transformed phenotype of the producer cell line. For biomarker validation studies, primary cell or clinical biofluid EV preparations are more relevant. Cell-line EVs are best suited to mechanistic and methods-development work.
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