RNA Methylation Analysis 

Comprehensive range of products for analysis of m⁶A and 5-mC RNA

RNA methylation occurs in different RNA species including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases. As a post-translational modification, RNA methylation plays a significant role as an epigenetic mechanism.

N6-methyladenosine (m⁶A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes. 5-methylcytosine (5-mC) also commonly occurs in various RNA molecules.

N6-methyladenosine (m⁶A)

Recent data strongly suggest that m⁶A and 5-mC RNA methylation affects the regulation of various biological processes such as RNA stability and mRNA translation, and that abnormal RNA methylation contributes to etiology of human diseases. Furthermore, loss of 5-mC in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Researchers continue to investigate m⁶A and 5-mC in RNA to uncover the mysteries of RNA methylation.

Global m⁶A & 5-mC RNA Quantification

EpigenTek's m⁶A and 5-mC RNA methylation kits make it easy for researchers to determine how RNA methylation influences their research project. The kits allow for the direct quantification of either N6-Methyladenosine (m⁶A) or measuring global 5-mC RNA using total RNA isolated from any species, including mammals, plants, fungi, bacteria, and viruses.

RNA m⁶A Methylase and Demethylase Activity

For measuring total m⁶A demethylase activity/inhibition using nuclear extracts or purified m⁶A demethylases like FTO and ALKBH5 from a broad range of species.

RNA Bisulfite Conversion & cDNA Synthesis

Bisulfite treatment techniques, also known as bisulfite conversion, are the most ideal approaches for preparing RNA for site-specific methylation analysis. This process converts cytosine into uracil, leaving methylated cytosine intact which is then easily distinguishable via high-throughput sequencing. Bisulfite converted RNA can also be used to generate cDNA for use in various downstream applications to analyze methylation condition. Besides the Bisulfit Conversion Kit and the cDNA Synthesis Kit EpigenTek also offers a complete kit for RNA bisulfite conversion and NGS library preparation for 5-methylcytosine RNA-seq.

Library Preparation for Next Generation Sequencing

Library preparation is a critical step for next generation sequencing (NGS). Many inexpensive solutions for NGS Library Preparation are offered, including kits that conveniently construct high-quality RNA/ DNA libraries from difficult samples that can be used in various Illumina-based platforms. 

MeRIP & m6A-Seq

Get high resolution mapping of m⁶A RNA using EpigenTek's  EpiQuik CUT&RUN m6A RNA (MeRIP) Enrichment Kit and EpiNext CUT&RUN RNA m6A-Seq Kit. With a unique nucleic acid cleavage enzyme mix, these kits isolate only RNA segments at the antibody binding locations and simultaneously cleave/remove the segments of RNA sequence just under the target area. Both our kits can enrich RNA fragments containing the m⁶A modification from low input RNA, with minimal non-specific background levels. The CUT&RUN RNA m6A-Seq Kit includes the library preparation step as an added benefit.

Antibodies to Study RNA Methylation

N6-methyladenosine (m⁶A) is one of the most common and abundant modifications found in RNA molecules present in eukaryotes. The chemical properties of m⁶A make it challenging to distinguish; therefore, methods to analyze this modification are highly dependent upon the quality of the ant-m⁶A antibody used. Both our polyclonal and monoclonal m⁶A antibodies can be reliably used for the specific detection of m⁶A in RNA. They have been thoroughly tested and are guaranteed to work in their respective applications.