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Protein Expression

Recombinant protein expression, from gene to purified protein, with yield guaranteed

Producing a recombinant protein should be one less thing to worry about. You take your target from gene to purified protein in E. coli, with gene synthesis, sequence validation, expression, and purification covered in one workflow. The overall success rate is above 95%, and the quantity and purity you agree are guaranteed. Target proteins of different sizes are supported for you.

How the workflow runs

  1. Construct. Codon optimisation, gene synthesis, cloning into an expression vector, and sequence validation. This step is optional if you already have a construct.
  2. Expression and optimisation. Small-scale tests find the best expression conditions for you before scale-up.
  3. Purification and scale-up. The protein is purified and produced at the quantity and purity you need, with optional tag removal.
  4. Quality control and delivery. Purity is checked by SDS-PAGE and Western blot, and quantity by Bradford, BCA, or A280.

Applications

Antigen and immunogen supply

Purified recombinant proteins act as antigens for antibody generation and as targets in binding assays.

Assay development

Defined proteins serve as standards and controls in ELISA, protein quantification, and enzyme assays.

Structural biology

Milligram quantities of pure protein support crystallography, cryo-EM, and biophysical characterisation.

Interaction and pathway studies

Tagged proteins enable pull-downs and interaction mapping, ready to pair with native and recombinant proteins.

Enzyme and functional work

Soluble recombinant enzymes drive kinetic studies, though bioactivity is not guaranteed and should be confirmed in your assay.

Custom constructs from scratch

You can start from a sequence with gene synthesis and codon optimisation when no construct exists.

Custom Protein Expression Pricing - Tagged and Tag-free Proteins

Quantity Purity Price - Tagged Protein* Price - Tag-free Protein
3 mg 85% 1620 € 3190 €
3 mg 90% 2070 € 4300 €
3 mg 95% 2515 € -
5 mg 85% 2180 € 4410 €
5 mg 90% 2670 € 5530 €
5 mg 95% 3080 € -
10 mg 85% 2740 € 6760 €
10 mg 90% 3190 € 8780 €
10 mg 95% 3630 € -

+Prices only apply if gene expression construct is available. Gene synthesis services are offered for an additional cost.

*Available tags: His, MBP (Maltose Binding Protein), GST (Glutathione-S-Transferase)

Contact BioCat

For detailed pricing, please simply send us your Protein Expression services request

Upon receipt we will closely inspect your project and send you a detailed quote right away. We typically respond within one business day.

Request a Quote
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Frequently asked questions

What protein sizes and tags are supported?

Target proteins between 10 kDa and 100 kDa are supported. Available tags are His, MBP, and GST, with optional tag removal as a final step.

 What is guaranteed, and what is not?

The agreed quantity and purity are guaranteed, and solubility is guaranteed. Bioactivity and functionality are not guaranteed and should be confirmed in your assay. If the protein forms inclusion bodies, refolding is attempted to make it soluble.
How is the protein delivered and checked?

The protein ships as lyophilised powder where possible, or in liquid form if solubility changes after lyophilisation. Purity is measured by SDS-PAGE and Western blot for tagged proteins, and quantity by Bradford, BCA, or A280. HPLC purity is available on request.

Product catalogs

Not sure which catalog to start with? We will help you find the right products.

Tell us what you are looking for — antibodies, kits, proteins, or supplies — and our team will point you to the best catalog filters, suppliers, and product matches for your workflow.

Get catalog help
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