Proteins
Picking the wrong protein format can cost you a repeat experiment. Native formats preserve physiological post-translational modifications and isoforms. Recombinant formats give you consistent batch-to-batch composition and scalable production.
Native proteins suit work where post-translational modifications and isoforms need to represent physiological conditions. Recombinant proteins suit your projects where you need tag-ready material that scales reliably.
These proteins support cell culture stimulation, functional assays, structural studies, and biochemical characterisation. You can choose from cytokines, growth factors, transcription factors, signalling proteins, and native proteins, across human, mouse, rat, and other species.
Proteins are produced in mammalian (HEK293, CHO), insect (Sf9, Hi5), yeast, and bacterial expression systems. Each system is matched to the structural and activity requirements of your target.
Mammalian systems such as HEK293 and CHO give closer-to-native glycosylation and disulfide processing. Glycosylation may still differ from the endogenous tissue. Check host and glycoform data on the CoA for your glycan-sensitive work.
Mammalian systems suit cell-based assays that need full biological activity. Bacterial expression suits applications where glycosylation does not matter and yield is your priority.
More than 14,000 human and mouse recombinant protein targets are available as His-tagged fusions, with purity confirmed by SDS-PAGE. Custom expression projects cover targets outside the standard range for your project.
Most proteins are supplied as lyophilised powder for long-term storage. They can be reconstituted in carrier-protein-free PBS for your bioassay use.
Antibody development and validation
Recombinant proteins serve as immunogens and as capture standards in ELISA and western blot, confirming assay specificity against a defined antigen.
Enzyme activity assays and inhibitor screening
Purified recombinant enzymes with defined specific activity serve as drug discovery targets, letting you measure IC50 values for inhibitors.
Structural biology
X-ray crystallography and cryo-electron microscopy need milligram quantities of bacterially or insect-expressed protein to examine binding interfaces and conformations.
Cell-based assays and cytokine stimulation
Endotoxin-free cytokines, growth factors, and ligands activate or inhibit signalling pathways in primary cells under defined conditions.
Biophysical binding characterisation
Untagged or site-specifically biotinylated formats support SPR and ITC experiments, measuring binding kinetics without tag interference.
Custom protein production for novel targets
For targets outside the standard range, custom expression pairs gene synthesis with purpose-built purification to match your needs.
Product Catalog
Native and Recombinant Proteins Matched to Your Assay for Reproducible Results
Native proteins are purified directly from their natural source tissue or organism. They retain any post-translational modifications present in that biological context. Recombinant proteins are made by expressing a cloned gene in a host such as E. coli, yeast, insect cells, or mammalian cells.
They are proteins that can be produced at consistent yield and quality. They also avoid contaminating proteins from the source organism.
E. coli gives the highest yields at the lowest cost. However, it does not glycosylate proteins and can form insoluble inclusion bodies for disulfide-rich or complex eukaryotic proteins. Insect cell systems using baculovirus support some glycosylation and disulfide bond formation.
Mammalian systems such as HEK293 and CHO can provide mammalian-like glycosylation and disulfide processing. This may matter for cytokines, growth factors, and receptor ectodomains. Glycosylation is not always identical to the endogenous tissue, disease state, or isoform. For glycan-sensitive applications, check host, glycoform data, and activity on the CoA or datasheet.
Lipopolysaccharide (LPS) endotoxin from E. coli outer membranes co-purifies with recombinant protein. It activates innate immune signalling through TLR4. Even low nanogram-per-millilitre levels can interfere with primary cell culture, cytokine readouts, and in vivo experiments.
Endotoxin-reduced or endotoxin-free grade protein is required for these applications. Verify this grade using the LAL or rFC assay.
The right activity test depends on the protein type. Enzymes are tested with a substrate turnover assay that measures product formation rate. Cytokines and growth factors are tested in a cell-based proliferation or signalling assay using a responsive cell line.
Binding proteins are verified by SPR or ELISA against a validated interaction partner. Express activity per milligram of protein to allow lot-to-lot comparison.
Product catalogs
Tell us what you are looking for — antibodies, kits, proteins, or supplies — and our team will point you to the best catalog filters, suppliers, and product matches for your workflow.
