Codon optimisation replaces codons in the target sequence with synonymous codons that match the tRNA abundance of the production host. In E. coli, rare codons such as AGA for arginine can stall ribosomes and reduce yield. Optimisation for the chosen host, whether bacterial, mammalian, insect, or yeast, typically increases expression level without changing the protein sequence.
Services
Custom Gene Synthesis Service with Codon Optimisation
Custom gene synthesis service delivering ready-to-use gene fragments and cloned constructs
Constructs range from short dsDNA fragments to fully cloned, sequence-verified plasmids, covering a wide range of lengths and complexities for your project. More than 160 standard vectors are available for cloning free of charge, and you can also send your own vector.
Turnaround and pricing are project-specific. They depend on sequence complexity and construct requirements. Gene fragment synthesis for uncloned dsDNA is available alongside full cloned gene synthesis. Site-directed mutagenesis of existing constructs is also available as an add-on service. All pricing is on request.
All plasmid DNA is delivered lyophilised at 4 micrograms minimum, research-grade purity. Higher purity grades and larger quantities are available for your application.
Projects cannot be initiated by phone. Sequences and order details must be submitted via the online gene synthesis request form or by email to info@biocat.com.
Scoping your synthesis project
- Which sequence or construct do you need? Identify your target: a full coding sequence, a fragment for downstream assembly, a regulatory element, or a mutagenised clone. This determines whether you need cloned gene synthesis, uncloned fragment synthesis, or site-directed mutagenesis.
- Fragment synthesis or gene synthesis plus cloning? If you need a stable, sequence-verified construct in a plasmid ready for transformation and expression, choose gene synthesis plus cloning. If you need uncloned linear dsDNA, choose fragment synthesis. Fragment synthesis is available for sequences of about 100 to 2000 bp in length.
- Which vector? More than 160 standard cloning and expression vectors are available at no extra charge. These cover bacterial, mammalian, baculovirus, insect, yeast, and plant systems. If your backbone is proprietary or not in the standard panel, you can supply your own vector. A one-time setup applies for the first order.
- Which cloning strategy? Choose the restriction or insertion sites for cloning the sequence of interest into the vector of choice. Cloning options include various restriction enzymes as well as seamless cloning for your construct. The BioCat team will advise on the appropriate strategy based on your sequence and vector.
- Is plasmid purification needed? The standard service delivers miniprep-grade plasmid DNA (4 micrograms minimum, lyophilised). Higher purity grades, endotoxin-free preparations, and larger quantities are available on request.
Services
Request Gene Synthesis
Please enter your sequence information and personal data. Upon receipt of your synthesis request, we will check your sequence and send you a detailed quote.
Applications
Antibody and protein engineering
Gene synthesis builds antibody fragments, bispecifics, and site-directed mutants without needing cDNA, with CHO or HEK293 codon optimisation included.
CRISPR tools and donor templates
Synthesised plasmids or dsDNA fragments provide homology-directed repair templates and ready-to-use gRNA cassettes, complementing your CRISPR-Cas9 reagents.
Pathway engineering and synthetic biology
Multi-gene, codon-optimised constructs compress design, build and test cycles versus sequential cloning, pairing with inducible expression vectors.
Assay controls, standards, and reporter constructs
Custom plasmids and gene fragments encoding standards, calibrators, and reporter fusions support your assay development and validation.
Probe, bait, and library generation
Short synthesised dsDNA fragments (typically 150 to 500 bp) serve as hybridisation probes, capture baits, and building blocks for ORF clone libraries.
Structural biology constructs
Truncated domains, deletion variants, and fusion-tagged constructs synthesise directly at your desired boundaries, avoiding multi-step subcloning.
Frequently asked questions
Standard synthesis covers sequences up to approximately 5 kb as a single construct. Longer sequences accumulate assembly errors during the oligo-ligation steps. Constructs above 5 kb are usually split into sub-fragments that are joined by a downstream sequence-independent assembly. Technical feasibility is assessed at the time of order for sequences approaching the upper limit.
A FASTA or plain nucleotide sequence is sufficient. If you also supply a GenBank or SnapGene file, it helps the team confirm reading frame, tag positions, and restriction sites. You should specify the intended expression host, the desired vector backbone, and any sequence elements to retain verbatim without codon alteration, such as existing tag sequences.
Yes. Gene fragments are uncloned linear dsDNA produced by oligo assembly. They are not sequence-verified individually and are used directly in downstream reactions such as sequence-independent assembly, homology-directed repair, or in vitro transcription. Cloned synthetic genes are sequence-verified in a plasmid vector and are ready for transformation and expression. Choose fragments when you need dsDNA input for assembly, and clones when you need a stable, confirmed expression construct.
Product catalogs
Not sure which catalog to start with? We will help you find the right products.
Tell us what you are looking for — antibodies, kits, proteins, or supplies — and our team will point you to the best catalog filters, suppliers, and product matches for your workflow.
