SKU: BIO-39038-BL
dCTP, 100mM
The ready-to-use molecular grade 100 mM solution of dCTP as lithium salts in purified water.
- High quality - >99% purity determined by HPLC, ideal for use in PCR reactions
- Long shelf-life - no need for aliquoting
- Convenient format – ready-to-use concentration
- Enzyme free - DNase, RNase and Nickase free
Description
Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dCTP undergo highly stringent purification steps to give a greater than 99% purity and are tested for the absence of DNase, RNase, Protease, Nickase activity. It is then quality controlled in a variety of applications, ensuring that it is highly suitable for the most sensitive techniques.
Bioline dCTP is supplied as lithium salts in purified water, as lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life.
Applications
- DNA amplification (standard, long-range, multiplex and high-fidelity PCR)
- RT-PCR
- qPCR
- Genotyping
- cDNA synthesis
- DNA sequencing
- DNA labeling
- Mutagenesis
- Expression profiling
- Epigenetic analysis
High quality deoxycytidine triphosphate (dCTP) in ready-to-use concentration
Two different batches of dNTPs were freeze-thawed 5, 10 and 20 times and two-fold serial dilution was used in a PCR reaction. Both batches produced identical PCR products of 2 kb whether freeze-thawed 5 times or freeze-thawed 20 times.