Air-Dryable 1-Step RT-qPCR Mix, 4x

Meridians Air-Dryable™ 1-Step RT-qPCR Mix is a glycerol-free mix and ideal for developing ambient temperature, air-dried, multiplex RT-qPCR molecular diagnostic tests. It is suited for high-throughput, automated platforms or microfluidic qPCR platforms. Can also be used wet.

• Create ultra-sensitive multiplex RT-qPCR tests for low-copy number targets
• Glycerol-free mixes are pre-formulated with a specialized blend of excipients, compatible with air-drying
• High-tolerance to PCR inhibitors carried over during purification, improving reproducibility
• Can be used wet or it can be dried, for developing an ambient-temperate stable assay
  
  

Molecular diagnostic tests are progressively moving towards dried formats. There are several advantages for this, including ambient temperature shipping and storage, extended shelf-life, reduced operating steps and potential errors and increased flexibility in sample volume. In order to be compatible with air or oven drying, however, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to higher temperatures.

Performance Data

Fig.1. Full enzyme activity following air-drying. Activity of Air-Dryable™ 1-Step RT-qPCR Mix in both wet (red) and air-dried (blue) formats were compared on 10-fold dilution mouse RNA template. The air-dried mix showed no loss of activity and sensitivity when compared to freshly prepared wet mix up to the assay limit of detection.