PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector

Single Promoter Expression Vectors for shRNA

Product Description

Leverage PiggyBac to produce shRNAs with this vector that co-express GFP and puromycin resistance from the EF1α promoter, and shRNA from the H1 promoter

• Make transgenic cell lines with a single transfection
• Integrate multiple PiggyBac Vectors in a single transfection
• Insert an expression cassette into human, mouse, and rat cells
• Deliver virtually any-sized DNA insert, from 10 – 100 kb
• Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers

Not just for genes, the PiggyBac system is also an excellent choice for reliably producing shRNA. The PB-EF1α-GreenPuro-H1-MCS shRNA Cloning and Expression Vector drives production of your shRNA from the strong H1 promoter. The vector also features GFP and puromycin resistance co-expressed from the moderate EF1α promoter, with co-expression mediated by the T2A element.