Super PiggyBac Transposase Expression Vector

Catalyze your PiggyBac genome engineering with this enhanced, hyperactive transposase that can insert any-length DNA segment into the genome

Product Description

Get easy, consistent transgenesis

The Super PiggyBac transposase expression vector features a strong CMV promoter to provide robust expression of the transposase. The PiggyBac transposase coding sequence has been optimized for high expression, stability and integration activity in mammalian cells.

The Super PiggyBac Transposase recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the genome at TTAA sites. The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors.

It is possible to reverse the integration reaction footprint-free with the Excision only PiggyBac Transposase Expression Vector.

The transposase plasmids are designed to use directly and cannot be propagated in bacteria.

Performance Data

Figure 1. Efficient transgenesis with the Super PiggyBac Transposase and both single- and dual-promoter PiggyBac Vectors. (Top four panels) Co-transfection with the Super PiggyBac Transposase Expression Vector and a Dual Promoter PiggyBac Cloning and Expression Vector (Cat.# PB513B-1-SBI) into HeLa cells demonstrates the efficient integration delivered by SBI’s PiggyBac Transposon System. After ten days of puromycin selection, only the cells co-transfected with the Super PiggyBac Transposase (+PB, right two panels) show robust growth and GFP fluorescence. (Bottom four panels) Co-transfection with the Super PiggyBac Transposase Expression Vector and a Single Promoter PiggyBac Cloning and Expression Vector (Cat.# PB531A-2-SBI) into HEK293 cells further demonstrates the efficient integration delivered by SBI’s PiggyBac Transposon System. After seven days of growth, the majority of cells that received the Super PiggyBac Transposase Expression Vector (+PB, right two panels) were RFP positive.

Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas Commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.