Excision only PiggyBac Transposase Expression Vector

Remove a PiggyBac insert from the genome and restore the original genomic sequence with this Excision-only PiggyBac Transposase

Product Description

Reversible transgenesis that leaves no trace behind

The Excision only PiggyBac transposase expression vector features a strong CMV promoter to provide robust expression of the transposase. The engineered PiggyBac Excision only transposase coding sequence has been optimized for transposon excision only activity and no transposon integration activity in mammalian cells.

With the Excision-only PiggyBac Transposase, delivered by transient transfection of the Exicison-only PiggyBac Transposase Expression Vector, you can remove any DNA integrated into the genome using the PiggyBac Transposon System. After removal of the PiggyBac insert, the genomic DNA is restored to the original sequence with no residual PiggyBac sequences left behind, resulting in a truly footprint-free removal.

Both the Excision-only PiggyBac Transposase and the Super PiggyBac Transposase recognize transposon-specific inverted terminal repeats (ITRs). While the Super PiggyBac Transposase has both excision and integration activities that enable the enzyme to mediate removal of the ITRs and intervening DNA from one location (the vector) and insert that DNA fragment into another location (the genome), the Excision-only PiggyBac Transposase only has the excision activity. Thus, the Excision-only PiggyBac Transposase can be used to remove DNA segments from the genome that are bordered by the PiggyBac transposon ITRs.

The transposase plasmids are designed to use directly and cannot be propagated in bacteria.

Performance Data

Reverse transgenesis with the PiggyBac Transposon System’s footprint-free excision process

Figure 1. SBI’s Excision-only PiggyBac Transposase is integrase-deficient but excision capable for reversible transgenesis. (Top panels) Comparison of the integration abilities of the Super PiggyBac Transposase to the Excision-only PiggyBac Transposase upon co-transfection with a GFP-expressing PiggyBac Vector. The very low number of GFP-positive cells in the sample co-transfected with Excision-only Transposase versus the numerous GFP-positive cells in the sample co-transfected with the Super PiggyBac Transposase demonstrate the poor integration abilities of the Excision-only enzyme. (Lower panels) We inserted a GFP expression cassette into the genome, and then inactivated GFP expression by disrupting the open reading frame with a PiggyBac transposon. Successful use of the Excision-only PiggyBac Transposase restores GFP function, leading to GFP-positive cells. Restoration of GFP fluorescence also demonstrates the seamlessness of excision—the GFP coding region would remain disrupted if the transposon sequences did not get completely removed.

Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas Commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.