PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector

Dual Promoter Expression Vectors for cDNA and microRNA

Product Description

This vector belongs to the PiggyBac Dual Promoter Vector Series. Vectors PB510B-1 to PB514B-2 carry the CMV promoter for high expression levels in most cell types, whereas vector PB713B-1 features the MSCV promoter which is active in stem cells.

The multiple cloning site (MCS) located downstream of the promoter allows for convenient cloning of your gene or microRNA of interest. Downstream of your expression cassette is an EF1alpha promoter driving the expression of either the Puro, GFP, RFP, GFP+Puro or RFP+Puro markers. The entire cassette is flanked by genomic insulator elements for stabilized expression and PiggyBac Inverted Terminal repeats for mobilization and integration.

The PiggyBac Transposon System consists of two parts

1. PiggyBac transposase expression vector (see below)
2. PiggyBac transposon vector of your choice

Related Products

Super PiggyBac Transposase Expression Vector
Excision only PiggyBac Transposase Expression Vector

Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas Commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology. 

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.