PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector

Robust, titratable Gene Expression delivered using the PiggyBac Transposon System

Product Description

• Make transgenic cell lines with a single transfection
• Integrate multiple PiggyBac Vectors in a single transfection
• Insert an expression cassette into human, mouse, and rat cells
• Deliver virtually any-sized DNA insert, from 10 – 100 kb
• Track insertion with GFP expression

More than just easy, consistent transgenesis with PiggyBac, the PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector (Cat.# PBQM812A-1) adds in the robust and titratable gene expression control of SBI’s cumate-inducible expression system. Clone your gene-of-interest into the MCS for cumate-inducible expression, which you can quantitatively monitor with the co-expressed GFP. This vector also co-expresses the cumate repressor, CymR, and puromycin resistance from the EF1α promoter.

• The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
• The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
• The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Tightly-controlled, inducible gene expression

Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.

The PiggyBac Transposon System consists of two parts

1. PiggyBac transposase expression vector (see below)
2. PiggyBac transposon vector of your choice

Related Products

Super PiggyBac Transposase Expression Vector
Excision only PiggyBac Transposase Expression Vector
Cumate Solution, high concentration
PiggyBac qPCR Copy Number Kit

Supporting Data

Figure 1. The PiggyBac cumate switch is titratable and can be turned off.

Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas Commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.