PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector

Single Promoter Expression Vectors for shRNA

Product Description

Leverage PiggyBac to produce shRNAs with this vector that co-express GFP and puromycin resistance from the CMV promoter, and shRNA from the H1 promoter

• Make transgenic cell lines with a single transfection
• Integrate multiple PiggyBac Vectors in a single transfection
• Insert an expression cassette into human, mouse, and rat cells
• Deliver virtually any-sized DNA insert, from 10 – 100 kb
• Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
• Determine the number of integration events with the PiggyBac qPCR Copy Number Kit

Not just for genes, the PiggyBac system is also an excellent choice for reliably producing shRNA. The PB-CMV-GreenPuro-H1-MCS shRNA Cloning and Expression Vector drives production of your shRNA from the strong H1 promoter. The vector also features GFP and puromycin resistance co-expressed from the strong CMV promoter, with co-expression mediated by the T2A element.