ExoGlow-RNA EV Labeling Kit

Product Description

Label EV mRNA (485 nm excitation/537 nm emission) with a reagent specifically optimized for EV labeling.

• Carefully developed to generate a robust signal specific for EV mRNAs, leading to very low levels of background
• Delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
• Easy-to-use labeling protocol is quick and straightforward
• Can be used with as little as 50 µg of EVs

Based on a highly specific polyT-fluorescent moiety conjugated to a stable peptidic backbone, the reagent specifically enters EVs and tightly binds mRNAs for fluorescence-based detection.

All ExoGlow EV Labeling Kits at a glance

ExoGlow™-Protein EV Labeling Kit (Red)

ExoGlow™-Protein EV Labeling Kit (Green)

ExoGlow™-Protein EV Labeling Kit (Blue)

ExoGlow™-RNA EV Labeling Kit

ExoGlow™-Membrane EV Labeling Kit

Supporting Data

Figure 1. ExoGlow-RNA enables clear visualization of labeled EVs being internalized by target cells. HEK293T EVs were labeled with ExoGlow-RNA and followed uptake by HEK293T cells. The fluorescence signal localizes to perinuclear and cytoplasmic regions, as would be expected for mRNA.

Figure 2. ExoGlow-RNA specifically labels mRNA. ExoGlow-RNA was used to label total RNA isolated from HEK293T cells, and then specifically depleted mRNA using polyT magnetic beads. The almost complete loss of signal in the mRNA-depleted sample demonstrates the specificity of ExoGlow-RNA dye for mRNA.