ExoGlow-Membrane EV Labeling Kit

Product Description

Label EV membranes (465 nm excitation/635 nm emission) with a reagent specifically optimized for EV labeling.

• Carefully developed to generate a robust signal specific for EV membranes, leading to very low levels of background
• Delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
• Easy-to-use labeling protocol is quick and straightforward
• Can be used with as little as 1 µg of EVs

Based on a highly specific membrane sensor, the reagent specifically labels intact EVs for fluorescence-based detection, while showing minimal background in an unbound state.

All ExoGlow EV Labeling Kits at a glance

ExoGlow™-Protein EV Labeling Kit (Red)

ExoGlow™-Protein EV Labeling Kit (Green)

ExoGlow™-Protein EV Labeling Kit (Blue)

ExoGlow™-RNA EV Labeling Kit

ExoGlow™-Membrane EV Labeling Kit

Supporting Data

Figure 1. ExoGlow-Membrane enables clear visualization of labeled EVs being internalized by target cells. 50 µg of HEK293T EVs were labeled with ExoGlow-Membrane and then uptake by HEK293T cells was observed. The evenly distributed fluorescence signal shows internalization of labeled EVs by the target cells and the distribution of EV membranes to cellular membranes.

Figure 2. ExoGlow-Membrane is specific for intact membranes. PBS, liposomes, liposomes pre-solubilized with detergent (0.05% Tx100), EVs, and lipoproteins were incubated with ExoGlow-Membrane. Only the samples with intact membranes—liposomes and EVs (blue)—show a high fluorescence signal, demonstrating ExoGlow-Membrane’s specificity for intact membranes.