ExoGlow-Protein EV Labeling Kit (Blue)

Product Description

Label EV proteins blue (403 nm excitation/454 nm emission) with a reagent specifically optimized for EV labeling.

• Carefully developed to generate a robust signal specific for internal EV proteins, leading to very low levels of background
• Delivers robust performance on EVs isolated using all methods tested—including ExoQuick®, ultracentrifugation, and column-based workflows
• Easy-to-use labeling protocol is quick and straightforward
• Can be used with as little as 200 µg of EVs

Based on an enzymatic conversion of the native, non-fluorescent dye to an activated fluorescent moiety when taken up by EVs, the ExoGlow-Protein dye can be used for most applications that require visualization of labeled EVs such as EV uptake studies.

All ExoGlow EV Labeling Kits at a glance

ExoGlow™-Protein EV Labeling Kit (Red)

ExoGlow™-Protein EV Labeling Kit (Green)

ExoGlow™-Protein EV Labeling Kit (Blue)

ExoGlow™-RNA EV Labeling Kit

ExoGlow™-Membrane EV Labeling Kit

Supporting Data

Figure 1. ExoGlow-Protein enables clear visualization of labeled EVs being internalized by target cells. HEK293T EVs were labeled with ExoGlow-Protein and followed uptake by HEK293T cells. The punctate fluorescence signal shows internalization of labeled EVs by the target cells.

Figure 2. Punctate staining pattern is EV-specific. The punctate staining pattern (top panel) is due to ExoGlow-Protein-labeled EVs. When ExoGlow-Protein is added directly to target HEK293T cells (bottom panel), only diffuse, non-specific staining is seen.

Figure 3. ExoGlow-Protein signal is stable for up to at least 7 days after administration of labeled EVs to cells.