Histone H3K4me3 Antibody: SNAP-ChIP Certified, CUTANA CUT&RUN Compatible

CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.

Product Description

This antibody meets EpiCypher's lot-specific SNAP-Certified™ criteria for specificity and efficient target enrichment in both CUT&RUN and ChIP applications. In CUT&RUN, this requires <20% cross reactivity to related histone PTMs determined using the SNAP-CUTANA™ K-MetStat Panel of spike-in controls (19-1002-EPC). High target efficiency is confirmed by consistent genomic enrichment at 500k and 50k starting cells. In ChIP, this requires <20% cross-reactivity to related histone PTMs and >5% of target input recovered as determined using the SNAP-ChIP^® K-MetStat Panel of spike-in controls (19-1001-EPC)

Figure 1: CUT&RUN genome wide enrichment
CUT&RUN was performed as above (Figure 1). Sequence reads were aligned to 18,793 annotated transcription start sites (TSSs, ± 2 kbp). Signal enrichment was sorted from highest to lowest (top to bottom) relative to H3K4me3 - 50k sample (all gene rows aligned). High, medium, and low intensity are shown in red, yellow, and blue, respectively. H3K4me3 antibody produced the expected TSS enrichment pattern that was consistent between 500k and 50k cells, while IgG negative control antibody (13-0042-EPC) displayed minimal background.

References

Grzybowski et al (2015) Mol Cell 58:886
Shah et al (2018) Mol Cell 72:162