Subcloning Services and Vector Cloning for Research

Custom cloning services for shRNA, sgRNA, and siRNA constructs, pooled libraries, and complex vector projects

Synthesis and cloning services in this category come from several suppliers. Describing what your construct has to do lets the scientific team in Heidelberg match you to the supplier whose service fits your budget and your quality control requirements.

Standard cloning and expression plasmids such as pET, pUC, and pcDNA are handled through the gene synthesis service. Custom cloning covers the more involved work: AAV constructs, minicircles, PiggyBac systems, and custom backbones, together with shRNA, sgRNA, and siRNA constructs and pooled shRNA and sgRNA libraries. Knowing which of the two routes your project belongs to shortens the conversation considerably.

Custom lentiviral and AAV cloning can be combined with a viral packaging service, so your construct is designed, built, and delivered as ready-to-transduce particles within one project. If you have found a plasmid on the site and want it combined with a synthesis and cloning service, the scientific team can tell you whether that combination is workable for your project and which supplier would handle it.

Named offerings in this category include the SYN2CLONE custom cDNA cloning service from System Biosciences; custom lentiviral shRNA and dual-shRNA expression constructs, and custom shRNA and sgRNA libraries from Cellecta; as well as cloning services covering highly customised lentiviral and AAV constructs from our partner Applied Biological Materials (abm). Formats, pack sizes, and quality control differ between them, and those details are confirmed for you with your quote.

How to choose a cloning route

1. Standard cloning and expression plasmids. Work built on pET, pUC, or pcDNA backbones belongs with the gene synthesis service, which covers routine expression constructs of the kind your standard workflows rely on.
2. Complex vector projects. AAV constructs, minicircles, PiggyBac systems, and custom backbones sit with custom cloning, where your design and assembly are scoped and quoted together.
3. Custom sgRNA cloning. For CRISPR knockout, CRISPRi, or CRISPRa work, sgRNA cloning spans sgRNA-only, all-in-one sgRNA-Cas9, dual-sgRNA, and inducible vectors. Two-vector and single-vector CRISPR-Cas9 systems are both available, with non-targeting and non-specific control guides in any backbone. Deliverables reach you as lentiviral plasmids or pre-packaged lentiviral particles.
4. Custom shRNA and siRNA cloning. Custom lentiviral constructs can express shRNA against any transcript, with a constitutive or tet-inducible H1 or U6 promoter and a choice of GFP, RFP, PuroR, BleoR, NeoR, or Hygro-HK markers. Three to five shRNA constructs against a given human or mouse gene can be supplied, designed on the parameters Cellecta uses for its own libraries. Custom cloning is offered for siRNA constructs as well, with formats for your targets confirmed by supplier at quotation.
5. Custom shRNA and sgRNA libraries. Pooled library work runs through Cellecta, which has more than fifteen years of experience building pooled shRNA, barcode, and sgRNA lentiviral libraries targeting any defined gene set. Libraries arrive in plasmid and pre-packaged, ready-to-transduce formats, in the constitutive or inducible vectors your screen calls for.