PiggyBac Transposon System
PiggyBac Transposon System
Easy, consistent transgenesis with no limits on cargo size. Footprint-free removal that leaves no PiggyBac sequences behind.
- Cut and paste transposition
- One transfection makes transgenic cell lines
- Effective in human, mouse and rat cells
- No cargo limit — integrate 10-100kb
- All-in-one tight inducible PiggyBac vector
The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the PiggyBac Transposon System* enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.
The unique features of PiggyBac transposons are that there is no cargo limit and it is also reversible. Genomes containing an inserted PiggyBac vector can be transiently re-transfected with the PB tranposase expression vector. The PB transposase will remove the transposons from the genome, footprint-free.
The PiggyBac Transposon System consists of two parts
- PiggyBac transposase expression vector (PB210PA-1-SBI or PB220PA-1-SBI)
- PiggyBac transposon vector of your choice
The PiggyBac all-in-one inducible vector (PBQM812A-1-SBI) will require the cumate solution (PBQM100A-1-SBI) for induction.
How it works
Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.
- Step 1 The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
- Step 2 The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
- Step 3 The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal.
Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas Commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology.
For end user license information, see the following:
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.