Services
Next Generation Sequencing Services for Research
Next generation sequencing services covering RNA, DNA and pooled screen readouts
Five sequencing services are grouped here. They cover targeted DNA and RNA sequencing services from Arbor Biosciences, sequencing of exosomal RNA cargo, small RNA and total RNA sequencing, as well as DNA sequencing of samples from pooled genetic screens. Which one suits your work depends on the molecule you want to read and on the material you have to send.
These services are set up to run end to end, so your samples go out and analysed data comes back. Sample requirements, read depth and analysis scope are settled with you before work starts, and pricing is available on request.
If you would rather build libraries at your own bench, the DNA library preparation or RNA library preparation range covers that step of the workflow which can be combined with hybrid capture kits from Arbor Biosciences.
mRNA-seq service covering library prep through differential expression analysis
When you do not have in-house NGS infrastructure, mRNA-seq services cover library prep through differential expression results. The service handles RNA QC, library prep, sequencing, and standard bioinformatics.
Pick by scope and turnaround.
- Scope. Library-prep-and-sequencing-only versus full bioinformatics through pathway analysis.
- Turnaround. Typically 4 to 8 weeks from sample receipt to results.
- Sample input flexibility. Verify low-input or FFPE compatibility for your samples.
- Bioinformatics deliverables. Confirm what the service includes (alignment, differential expression, pathway analysis).
- Use case. For one-off projects outsourcing is cost-effective. For ongoing transcriptomics in-house capability pays back at scale. BioCat provides mRNA-seq services in this inventory.
DNA-seq service for pooled CRISPR and shRNA screen library prep and analysis
When you have run a pooled CRISPR or shRNA screen and need to convert genomic DNA into a barcode-count table, this service handles library prep, sequencing, and analysis deliverables. Removes the in-house bottleneck of screen-specific NGS workflow setup.
Pick by screen and PCR strategy.
- Screen format. Indexing primer set must match the lentiviral backbone of the library. Mismatch leads to no amplification.
- PCR strategy. One-step PCR with indexing primers is fastest. Two-step PCR adds index purity and lowers PCR-bias for low-input samples.
- Library quantification. qPCR-based gives more accurate sequencing-pool ratios than fluorometric or Bioanalyzer methods.
- Coverage. For low-input or rare-event positive-selection screens use two-step PCR to retain library complexity.
- Indexing scheme. UDI for low cross-talk at scale. Cellecta supplies library-matched reagents for DECIPHER screen platforms.
Small RNA-seq library prep kits for microRNA, piRNA, and other short fragments
When you need transcriptome-wide microRNA, piRNA, tRNA fragment, snRNA, or degradation fragment quantification, small RNA-seq library prep handles the short fragments (15 to 200 nt) that mRNA-seq misses. Specialised adapter ligation, gel-based size selection, and bias-mitigation chemistry differ from standard library prep.
myReads DNA and RNA sequencing service for standard and challenging samples
The myReads service from Daicel Arbor runs your NGS project end to end, from extraction through analysis. Pre-designed packages cover common DNA and RNA applications, and you can add myBaits hybridisation capture or customise steps for your sample type. The team handles difficult inputs such as degraded DNA and complex genomes, so you get usable data without becoming an NGS specialist.
Contact BioCat
Questions? We are here to help. Sales, orders, and general inquiries.
Reach our team for quotes, order status, shipping questions, or anything else related to working with BioCat. We typically respond within one business day.
