Small RNA-seq is particularly well suited for low-input and challenging sample types, enabling reliable profiling of miRNAs and other small RNAs from cells, tissues and biofluids such as plasma and serum. For broader transcriptome profiling, total RNA-seq and mRNA-seq services can be used to investigate messenger RNA, long non-coding RNA and circular RNA across a wide range of sample types, including biofluids. The most suitable workflow depends on your sample type, RNA quantity and research objective. For extracellular-vesicle projects, tailored workflows can support the analysis of RNA cargo alongside comprehensive extracellular-vesicle characterisation.
Services
Next Generation Sequencing Services for Research
Next generation sequencing services covering RNA, DNA and pooled screen readouts
Five sequencing services are grouped here. They cover targeted DNA and RNA sequencing services from Arbor Biosciences, sequencing of exosomal RNA cargo, small RNA and total RNA sequencing, as well as DNA sequencing of samples from pooled genetic screens. Which one suits your work depends on the molecule you want to read and on the material you have to send.
These services are set up to run end to end, so your samples go out and analysed data comes back. Sample requirements, read depth and analysis scope are settled with you before work starts, and pricing is available on request.
If you would rather build libraries at your own bench, the DNA library preparation or RNA library preparation range covers that step of the workflow which can be combined with hybrid capture kits from Arbor Biosciences.
Choosing the right sequencing service for your project
- Exosome RNA Next Generation Sequencing Service. This is the service to ask about when the RNA you care about travels inside exosomes and other extracellular vesicles. It belongs with the wider extracellular vesicle and exosome range, and the input, read depth and analysis specification for your samples is confirmed on enquiry.
- miRNA-seq and Small RNA-seq Services. Gain reliable insights into miRNAs and other small RNAs from a broad range of sample types, including cells, tissues and biofluids such as plasma and serum. Quality-controlled workflows and dedicated bioinformatic analysis provide robust, biologically meaningful results, with options for absolute miRNA quantification.
- Total RNA-seq and mRNA-seq Services. Explore the full transcriptome of your samples with mRNA and whole-transcriptome sequencing services covering messenger RNA, long non-coding RNA and circular RNA. From RNA isolation and quality control through library preparation, sequencing and bioinformatic analysis, tailored workflows deliver robust results for a broad range of biological sample types, including tissues, cells and biofluids.
- myReads Services from Arbor Biosciences. Arbor Biosciences, part of Daicel Corporation, runs the myReads packages, which bundle library preparation, optional hybridisation capture with myBaits, and sequencing. Pre-designed formats cover your standard, degraded, ancient, long-insert and RNA samples, and whole genome tiers run from 0.5 to 100 Gbp per sample.
- DNA-seq Service for samples from genetic screens. Frozen cell pellets, tissue or genomic DNA from your pooled CRISPR, shRNA and barcode screens are accepted, and a report comes back giving the number of reads per guide, hairpin or barcode. All Cellecta libraries are covered, along with other pooled lentiviral libraries such as Brunello and GecKO.
Applications
Circulating biomarker discovery
Small RNA sequencing from your plasma or serum picks up miRNA signatures, and the low-bias chemistry keeps rare species visible in the data.
Transcriptome profiling
Messenger RNA sequencing quantifies expression across your treatment groups and time points, feeding straight into transcriptome profiling studies.
Exosomal RNA cargo analysis
Sequencing the RNA carried by vesicles extends your extracellular vesicle workflow from isolation through to expression data.
Pooled screen deconvolution
Read counts per guide, hairpin or barcode turn your CRISPR or shRNA screen into ranked hits, with the downstream NGS handled end to end.
Capture on difficult specimens
myReads packages pair myBaits capture with sequencing for degraded, ancient and long-insert DNA, so awkward material in your collection still works.
Whole genome sequencing at set depths
Arbor packages are tiered by data volume per sample, from 0.5 Gbp for small bacterial genomes up to 100 Gbp for your vertebrate-scale work.
mRNA-seq service covering library prep through differential expression analysis
When you do not have in-house NGS infrastructure, mRNA-seq services cover library prep through differential expression results. The service handles RNA QC, library prep, sequencing, and standard bioinformatics.
Pick by scope and turnaround.
DNA-seq service for pooled CRISPR and shRNA screen library prep and analysis
When you have run a pooled CRISPR or shRNA screen and need to convert genomic DNA into a barcode-count table, this service handles library prep, sequencing, and analysis deliverables. Removes the in-house bottleneck of screen-specific NGS workflow setup.
Pick by screen and PCR strategy.
Small RNA-seq library prep kits for microRNA, piRNA, and other short fragments
When you need transcriptome-wide microRNA, piRNA, tRNA fragment, snRNA, or degradation fragment quantification, small RNA-seq library prep handles the short fragments (15 to 200 nt) that mRNA-seq misses. Specialised adapter ligation, gel-based size selection, and bias-mitigation chemistry differ from standard library prep.
myReads DNA and RNA sequencing service for standard and challenging samples
The myReads service from Daicel Arbor runs your NGS project end to end, from extraction through analysis. Pre-designed packages cover common DNA and RNA applications, and you can add myBaits hybridisation capture or customise steps for your sample type. The team handles difficult inputs such as degraded DNA and complex genomes, so you get usable data without becoming an NGS specialist.
Contact BioCat
For detailed pricing, please simply send us your NGS Sequencing services request
Upon receipt we will closely inspect your project and send you a detailed quote right away. We typically respond within one business day.
Product Catalog
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mRNA-seq Service
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DNA-seq Service (Genetic Screens)
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myRead Sequencing Service
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Frequently asked questions
The sequencing services support a wide range of research samples, from cells and tissue to challenging liquid-biopsy materials such as whole blood, plasma, serum and other biofluids. For targeted DNA sequencing, Arbor Biosciences' myReads panels provide flexible solutions for DNA samples and can be tailored to the genomic targets and species relevant to your study. Species compatibility depends on the selected assay, the availability of a suitable reference genome and the scientific question. RNA sequencing services are also available, including small RNA-seq, total RNA-seq and broader transcriptome studies, with options for biofluid and extracellular-vesicle projects. Sharing your sample type, species and target region allows the most appropriate workflow to be recommended.
You send frozen cell pellets, tissue or genomic DNA, and a report comes back with the number of reads per guide, hairpin or barcode from the pooled CRISPR, shRNA or barcode library used in the screen.
Sequencing covers all Cellecta libraries and a range of other pooled lentiviral libraries, including Brunello and GecKO.
Arbor Biosciences runs short-read work on the AVITI platform from Element Biosciences, which accepts Illumina libraries and returns the same FASTQ output format. Long-insert projects move to PacBio. Short-insert libraries carry 8 bp unique dual indexes and long-insert libraries carry 16 bp identical dual indexes.
Documented ethics approval is required for human samples, clinical specimens are not accepted, and Arbor is not CLIA-certified. Permits for shipments such as CITES-listed specimens or plant tissue regulated by USDA APHIS are the responsibility of the customer. Arbor states that it does not offer specific guarantees on turnaround time, unique-read coverage depth or on-target percentage, given how variable custom capture and sequencing work is.