SKU: TP302777-OR
TDO2 (NM_005651) Human Recombinant Protein
Human
In Stock
Dry Ice
Specifications
| Specification | Details |
|---|---|
| Molecular Weight | 47.7 kDa |
| Tag | C-terminal MYC/DDK |
| Source | HEK293 cells |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Activity | The specific activity of TDO2 was determined by monitoring Kynurenine formation from the N-formylkynurenine based on the absorbance at 492nm. The N-formylkynurenine was produced from a conversion of tryptophan with TDO2. The reactions were carried out at 37°C for 40min in 100ul of the reaction volume containing 200mM PBS, pH7.5, 1mM ascorbic acid, and 1.25mM L-tryptophan as the substrate with various amounts of TDO2. The reaction was terminated by adding 50ul of 30% (w/v) trichloroacetic acid. The sample was further incubated for 30min at 60°C and centrifuged at 12000 rpm for 15 min. The supernatant was used to mix with an equal volume of Ehrlich's reagent (2% p-dimethylaminobenza-ldehyde in glacial acetic acid) to measure the absorbance at 492 nm after 10min incubation |
| Synonyms | TDO; TO; TPH2; TRPO |
| Organism | Human |
| Accession No. | NP_005642 |
| Background | Recombinant protein of human tryptophan 2,3-dioxygenase (TDO2) |
Resources
PDF
Datasheet
Catalog Number
TP302777-OR
Name
TDO2 (NM_005651) Human Recombinant Protein
Price(2000 ug)
Pack Size
2000 ug