SKU: PKSM040307-50ug-ELS
Recombinant Mouse Legumain/LGMN Protein (His Tag)(Active)
Mouse
In Stock
RT
Specifications
| Specification | Details |
|---|---|
| Molecular Weight | 49.8 kDa |
| Tag | C-His |
| Source | HEK293 Cells |
| Purity | > 75 % as determined by reducing SDS-PAGE. |
| Endotoxin Level | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Activity | Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC).The specific activity is > 350 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH) |
| Format | Lyophilized from sterile 25mM Tris, 0.15M NaCl, 20% Glycerol, pH 7.5 |
| Synonyms | Legumain;Lgmn;Asparaginyl endopeptidase;Protease cysteine 1;Prsc1;AEP |
| Organism | Mouse |
| Accession No. | NP_035305.1 |
| Sequence Info | Val 18-Tyr 435 |
| Background | The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with a strict specificity for hydrolysis of asparaginyl bonds. Known previously only from plants and invertebrates, Legumain is discovered as a lysosomal endopeptidase in mammals. Mammalian Legumain is a cysteine endopeptidase, inhibited by iodoacetamide and maleimides, but unaffected by compound E64. The Mammalian Legumain is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Legumain has been observed to be highly expressed in several types of solid tumors. It was demonstrated in membrane-associated vesicles concentrated at the invadopodia of tumor cells and on cell surfaces where it colocalized with integrins. Legumain was demonstrated to activate progelatinase A. Cells overexpressing Legumain possessed increased migratory and invasive activity in vitro and adopted an invasive and metastatic phenotype in vivo, inferring significance of Legumain in tumor invasion and metastasis. In addition, Legumain is expressed in both murine and human atherosclerotic lesions. The macrophage-specific expression of Legumain in vivo and ability of Legumain to induce chemotaxis of monocytes and endothelial cells in vitro suggest that Legumain may play a functional role in atherogenesis. |
Resources
PDF
Datasheet
Catalog Number
PKSM040307-50ug-ELS
Name
Recombinant Mouse Legumain/LGMN Protein (His Tag)(Active)
Price(5000 ug)
Pack Size
5000 ug