MyTaq Red DNA Polymerase

• Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
• Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
• Sensitive – exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
• Flexible – ideal for amplifying any target up to 5 kb, including DNA extracted from human, animal and plant samples
• Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product

MyTaq™ Red DNA Polymerase is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Red Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates.

MyTaq has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq means it gives excellent results under fast PCR conditions.

The enzyme is supplied with a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq Red Reaction Buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

Applications

• Standard PCR
• High-yield PCR
• Fast PCR
• Colony PCR
• TA cloning

Highly efficient and robust amplification under a broad range of PCR conditions

MyTaq was compared with DNA polymerases form others suppliers for the amplification of a 450 bp fragment of the human myc gene (61% GC rich). Decreasing amounts of human genomic DNA were used as template (1 mg, 200 ng, 100 ng, 50 ng, 25 ng and 12.5 ng; lanes 1-6 respectively) in the PCR. Marker is HyperLadder 1kb (M). MyTaq delivers higher yield and sensitivity compared to five polymerases from alternative suppliers.

MyTaq DNA Polymerases Customer Review