Lyo-Ready Direct DNA LAMP Blood, 4x

Lyo-Ready™ Direct DNA LAMP Blood mix is a ready-to-use, 4x concentrated, fully optimized mix for isothermal amplification methods such as Loop mediated isothermal amplification (LAMP) on blood, designed to dramatically speed up assay development time and reduce time to results. The glycerol-free mix contains optimized excipients compatible with freezing and lyophilization, making it ideal for POC blood testing. Can also be used wet and/or with purified nucleic acid.

Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, whole blood specimens, serum, and plasma contain a number of inherent PCR inhibitors including immunoglobulin G, hemoglobin, lactoferrin, and leukocyte DNA. In addition, PCR inhibitors can be found in the anticoagulants used to stabilize blood samples (e.g. EDTA, citrate, or heparin). Isothermal amplification such as LAMP is very tolerant to inhibitors and so is ideal for point of care (POC) testing, the simple workflow allows fast, sensitive and reliable results in clinical diagnosis.

The Lyo-Ready™ Direct DNA LAMP Blood mix combines even greater tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crudely processed whole blood, plasma, or serum samples. The mix is uniquely designed to overcome much higher concentrations of inhibitors and so does not require a DNApurification step to concentrate and purify a patient sample. Very fast reaction kinetics result in faster time to results and superior amplification sensitivity and specificity. In addition, the 4x concentrated master mix is formulated with lyo-excipients which are optimized for freeze-drying to create ambient-temperature stable assays, making it ideal for POC blood testing. Please refer to the Lyophilization & Post-Lyophilization User Guideline for optimal usage.

Performance Data

Fig.1. Specifity and quicker time to detection for viral and bacterial targets. 
(A) Lyo-Ready™ Direct DNA LAMP Blood (MDX124, red) was lyophilized with primers for Trichomonas vaginalis, Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Epstein-Barr virus (EBV), Mycoplasma pneumoniae and
(B) Lyo-Ready™ Direct RNA/DNA LAMP Blood (MDX125, red) was lyophilized with primers for Hepatitis C Virus (HCV), HIV-1, Dengue type 1, West Nile Virus (WNV) or Zika.
The performance of the mixes were compared against NEB WarmStart® LAMP kit (orange) and Thermo SuperScript™ IV RT-LAMP Master Mix (black) using the same primers and DNA/RNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. Both Lyo-Ready™ Direct LAMP Blood mixes delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longer delay in no-template control (NTC) amplification across range of pathogenic DNA and viral RNA targets.