SKU: TP317924-OR
HNF 4 alpha (HNF4A) (NM_178850) Human Recombinant Protein
Human
Dry Ice
Specifications
| Specification | Details |
|---|---|
| Molecular Weight | 46.4 kDa |
| Tag | C-terminal MYC/DDK |
| Source | HEK293 cells |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Activity | HNF4A Activity Verified in a DNA-binding Assay: HNF4A (TP317924, transcript variant 3) activity was measured in a colorimetric DNA-binding assay. Purified HNF4A protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the HNF4A consensus DNA-binding sequence (see below). Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence. |
| Synonyms | FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14 |
| Organism | Human |
| Accession No. | NP_849181 |
| Background | Recombinant protein of human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 3 |
Resources
PDF
Datasheet
Catalog Number
TP317924-OR
Name
HNF 4 alpha (HNF4A) (NM_178850) Human Recombinant Protein
Price(20 ug)
Pack Size
20 ug