Elab Fluor® 488-11-dUTP

Chromosome DNA breakage is an important marker event in cell apoptosis. A series of DNA 3'-OH terminus is produced by DNA double-strand breaks in apoptotic cells or whenever there is a gap in one strand. The fluorescen labeled dUTP can be linked to the 3' -OH terminus of the broken DNA under the action of terminal deoxyribonucleotide transferase (TdT). Fluorescence of dUTP conjugate can be detected by flow cytometer or fluorescence microscope.

Performance Data

Fig.1. Paraffin embedded mouse colon was treated with DNase I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNase I treated sample(Green)