CRISPRuTest-EF1L Functional Cas9 iPSC Compatible Fluorescent Assay Kit for Mammalian Cells

The kit can be used to measure the Cas9 nuclease activity in most mammalian cell systems. The choice of kit (CRISPRuTest™ or CRISPRuTest™ (EF1L)), depends on the cell line to be tested. In iPS cells, where the CMV promoter used in the CRISPRuTest™ Kit is quickly silenced, the CRISPRuTest™ Kit (EF1L) is strongly recommended.

The CRISPR system uses a target single-guide RNA molecule (sgRNA) to target and cut a specific sequence on the genomic DNA with the Cas9 nuclease. The CRISPRuTest Kits provide FACS-based assays to assess the Cas9 nuclease activity in cells expressing Streptococcus pyogenes Cas9(spCas9) or Staphylococcus aureus_Cas9 (saCas9).

The CRISPRuTest™-EF1L Kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene whose fluorescence is disrupted in cells with active Cas9. The kit provides enough reagents to measure this activity in 5-15 cell lines.