Cas9 & dCas9 Activity Assays

Cas9 and dCas9 activity assays for nuclease cleavage and binding kinetics

When you need to characterise Cas9 nuclease cleavage activity or dCas9 binding kinetics in vitro, this section covers the assays. Fluorescently labelled or biotinylated DNA targets paired with gel-based, capillary-electrophoresis, or fluorescence-quench readouts let you profile guide RNA performance and characterise Cas9 protein lots.

Pick by readout.

1. Visual confirmation. Choose gel-based assays.
2. Kinetic monitoring. Choose fluorescence-quench readouts on a plate reader.
3. Fragment-size resolution. Choose capillary electrophoresis.
4. Substrate. Matched-target for activity baseline. Mismatch substrates for specificity profiling.
5. Controls. For lot QC always include matched-target positive control and unguided dCas9 negative control.

Cellecta supplies activity-assay kits in this inventory.