CRISPRuTest Functional Cas9 Activity Assay Kit for Mammalian Cells (for 5-15 cell lines)

Product Description

The CRISPRuTest™ Functional Cas9 Activity Assay Kit can be used to measure the Cas9 nuclease activity in most mammalian cell systems. The choice of kit (CRISPRuTest™ or CRISPRuTest™ (EF1L)), depends on the cell line to be tested. In iPS cells, where the CMV promoter used in the CRISPRuTest™ Kit is quickly silenced, the CRISPRuTest™ Kit (EF1L) is strongly recommended.

The CRISPR system uses a target single-guide RNA molecule (sgRNA) to target and cut a specific sequence on the genomic DNA with the Cas9 nuclease. The CRISPRuTest Kits provide FACS-based assays to assess the Cas9 nuclease activity in cells expressing Streptococcus pyogenes Cas9(spCas9) or Staphylococcus aureus_Cas9 (saCas9).

The CRISPRuTest™ Kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene whose fluorescence is disrupted in cells with active Cas9. The kit provides enough reagents to measure this activity in 5-15 cell lines.