PinPoint-FC 293T Platform Cell Line for Targeted Gene Insertion (with PinPoint site already placed)

Making the Two-step PinPoint Process a Single, Easy Step

Product Description

Jump start your PinPoint cell line creation with this 293T cell line that already has a single PinPoint attP site inserted into the genome

With the PinPoint-FC 293T Platform Cell Line for Targeted Gene Insertion you can skip the first step of the PinPoint Targeted Integration workflow because SBI’s already done it for you. This cell line comes with a PinPoint attP site already placed and validated to be present in single copy, so you can simply co-transfect with the PinPoint Integrase Expression Vector and an appropriate PinPoint Donor Vector. SBI also offers the convenient PinPoint-FC 293T Platform Kit for Targeted Gene Insertion, which includes this cell line and both vectors together with a positive control donor vector.

With this cell line, generating isogenic cell lines becomes a straightforward, one-step process that can be easily implemented as a high-throughput assay.

How it works

The PinPoint Integrase mediates a recombination event between the PinPoint attB site (located on the PinPoint Vector) and the PinPoint attP site. Because the PinPoint Integrase only recognizes the pre-placed PinPoint attP site, you get very stringent control of integration location.

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