PinPoint Donor Positive Control Vector: CAG->GFP+Luciferase (pPP-CAG-GFP-T2A-Luc-WPRE-pA-attB-Puro-pA)

Easy and Efficient Isogenic Cell Line Creation

Product Description

Use this GFP and Luciferase-expressing PinPoint Donor Vector as a positive control or to troubleshoot your PinPoint Targeted Integration Projects.

• Create isogenic cell lines
• Achieve high-efficiency integration with no insert size-limit
• Turn cell line construction into a high-throughput process

With the PinPoint Targeted Integration System you can get controlled, single-copy integration of your PinPoint Donor Vector at a pre-placed PinPoint attP site. The CAG-GFP-T2A-Luc PinPoint Positive Control Donor Vector (pPP-CAG-GFP-T2A-Luc-WPRE-pA-attB-Puro-pA) inserts GFP and luciferase markers into the PinPoint attP site. The markers are co-expressed from the strong CAG promoter, with co-expression mediated by a T2A element. The vector also includes a promoterless puromycin marker to enable selection for the desired integrants—proper integration into a PinPoint attP site inserted using SBI’s PinPoint Placement Vector results in a PGK promoter positioned to drive the puromycin marker, enabling selection for the desired integrants. The vector also includes a LoxP site to mediate removal of most of the unnecessary vector sequences and both kanamycin and hygromycin markers for cloning and propagation.

With the PinPoint Targeted Integration System, you can ensure that only a single copy of your transgene is inserted into the genome.

Learn more about Targeted integration with the PinPoint System