pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector

One-step, non-viral, single-copy transgenesis

Product Description

The pFC-EF1-MCS- PGK-RFP-Puro vector has EF1a driving your gene´s expression cloned into the MCS and PGK expressing the RFP-Puro combo markers. The bidirectional promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter. In addition to enabling coordinated expression of your gene-of-interest and a marker, this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells¹. 

Combine the advantages of the PhiC31 Integrase System with a bidirectional promoter format for your genome engineering projects with the pFC-EF1α-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector. Simply co-transfect with the PhiC31 Integrase Expression Plasmid and select for integrants using puromycin resistance and imaging for RFP fluorescence.

Alternative dual-promoter vector:

pFC-PGK-MCS-EF1-GFP-Puro utilizes PGK to express your gene cloned into the MCS and has EF1a driving the GFP-Puro combo markers. Both PGK and EF1a promoters are well-validated to have a broad range of activity across many cell types, including stem cells.