ExoQuick-LP for lipoprotein pre-clear & exosome isolation

Product Description

• Efficiently pre-clear serum or plasma lipoproteins before exosome isolation

For researchers who specifically want to study exosomal RNA or proteins, removing non-exosomal molecules prior to treatment with ExoQuick^® can lead to data that more reliably reports on exosomal RNA or proteins. Because both high density and low density lipoproteins (HDLs and LDLs) can contain miRNAs¹, lipoprotein removal is recommended prior to exosome isolation from serum or plasma. ExoQuick-LP^® is specifically designed to remove normally-occurring lipoprotein particles from serum or plasma prior to ExoQuick precipitation of exosomes.

The ExoQuick-LP Kit contains ExoQuick and proprietary pre-clearing reagents that efficiently deplete lipoprotein particles from plasma or serum prior to ExoQuick treatment.

Performance Data

With ExoQuick-LP, up to 90% depletion of lipoprotein particles from serum or plasma can be achieved. SBI’s exosome isolation products help you take full advantage of the power of exosomes.

ExoQuick-LP efficiently clears lipoproteins from serum

ExoQuick-LP treatment of serum results in lower amounts of lipoprotein markers (ApoA-1 for HDLs, and ApoE for LDLs) that co-isolate with exosomes than treatment of serum with ExoQuick alone, as shown by ELISA.

Figure 1. ExoQuick-LP efficiently clears lipoproteins from serum.

Exosome recovery comparisons and NanoSight size analysis

To test for exosome recovery after ExoQuick-LP pre-clearance, SBI's Exosome ELISA Kits (Cat. # EXOEL-CD81A-1-SBI, EXOEL-CD63A-1-SBI, and EXOEL-CD9A-1-SBI) were used to measure the approximate number of exosomal particles present in ExoQuick serum pellets with or without treatment with ExoQuick-LP Pre-Clearing Reagents. While there were some differences in observed particle numbers in untreated vs treated samples, overall yields were quite similar, suggesting minimal loss of material using ExoQuick-LP.

Figure 2. ExoQuick-LP delivers robust exosome yields and particles of the size expected for exosomes.

Serum exosomes pre-treated with ExoQuick-LP were tested for size and concentration using NanoSight Tracking Analysis with a 1:100 dilution of isolated exosomes. It was observed that there are minimal differences in particle sizes using ExoQuick-LP vs untreated controls, suggesting that exosomes are structurally intact similar to using standard ExoQuick isolation methods.

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