ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
Specifications | |
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Product Category: | Exosome Purification |
Sample Type: | Plasma/Serum |
Product Description
The revolutionary new ExoQuick® ULTRA is our most advanced polymer-based method for isolating Extracellular Vesicles, resulting in 420X more EVs than ultracentrifugation.
- Even cleaner—reduces carry-over of albumins by 75% and immunoglobulins by 40% more than other methods, including ultracentrifugation and competitors’ kits
- Higher yields—isolate more EVs per normalized input volume than ultracentrifugation and competitors’ kits
- Better biomarker detection—see what you’ve been missing when you increase the sensitivity of EV biomarker detection by up to 11-fold (CD9) and 8-fold (CD81)
- Fast—requires less than 20-minutes of hands-on time, including a convenient column-based format for clean-up
- Cost-effective—save money with each reaction compared to how much you’ll spend using a competitor’s kit
From as little as 250 uL of serum or plasma using the ExoQuick ULTRA kit, you can isolate high-quality EVs for a wide range of downstream applications such as Western blot analysis, mass spectrometry, NGS sequencing, exosome labeling, and in vivo/ex vivo exosome delivery.
To isolate exosomes from plasma, we recommend pre-treatment with the Thrombin Plasma Prep or using the ExoQuick™ Plasma Prep and Exosome Precipitation Kit.
How it works
The ExoQuick ULTRA protocol is quick and easy, requiring only 10-minutes of hands-on time:
Fig.1. ExoQuick ULTRA Workflow
Performance Data
Figure 2. ExoQuick ULTRA delivers high yields of clean exosomes. (A) A coomassie blue-stained protein gel comparing the protein content of exosome preps isolated using different methods shows only a few, defined protein bands in the ExoQuick ULTRA lane compared to the other methods. (B) Western blotting of the gel shows that the ExoQuick ULTRA prep contains the highest levels of exosome-specific markers CD9, CD81, and Hsp70 and the lowest levels of the carryover proteins albumin and IgGH. In contrast, the prep from Company Q appears to be primarily albumin, and even the sample prepared using ultracentrifugation contains considerably higher levels of both albumin and IgGH. Each lane was loaded with 7 ug of total protein as measured using a fluorometric Qubit protein assay.
More data of performance can be found in the ExoQuick ULTRA Product Sheet.
- Catalog Number
EQULTRA-20A-1-SBI - Supplier
SBI System Biosciences - Size
- Shipping
Blue Ice