ExoQuick-TC ULTRA EV Isolation Kit for Tissue Culture Media

Product Description

Leveraging their years of exosome expertise, the SBI team has pushed ExoQuick-TC extracellular vesicle (EV) isolation technology to new peaks of performance with ExoQuick-TC ULTRA for tissue culture media and most biofluids. While many EV isolation methods force you to choose between high yields, easy protocols, clean preps, and low costs, ExoQuick-TC ULTRA is able to deliver on all these fronts for trade-off free EV preparation.

• Even cleaner—reduces carry-over of albumins by 75% and immunoglobulins by 40% more than other methods, including ultracentrifugation and competitors’ kits
• Higher yields—isolate more EVs per normalized input volume than ultracentrifugation and competitors’ kits
• Better biomarker detection—see what you’ve been missing when you increase the sensitivity of EV biomarker detection by up to 11-fold (CD9) and 8-fold (CD81)
• Fast—requires less than 20-minutes of hands-on time, including a convenient column-based format for clean-up
• Cost-effective—save money with each reaction compared to how much you’ll spend using a competitor’s kit

How it works

The ExoQuick-TC ULTRA protocol is quick and easy, requiring only 10-minutes of hands-on time: 

Fig.1. ExoQuick ULTRA Workflow 

Performance Data

Figure 2. ExoQuick-TC ULTRA delivers high yields of clean exosomes. Comparison of different EV isolation methods via Western blot shows that the ExoQuick-TC ULTRA prep contains the highest levels of the exosome-specific marker CD63 and the lowest levels of the carryover protein albumin. In contrast, the prep from Company Q appears to be primarily albumin, and even the sample prepared using ultracentrifugation contains considerably higher levels of both albumin and IgGH. Each lane was loaded with 4 ug of total protein. 

More data of performance can be found in the ExoQuick ULTRA Product Sheet.