Proteospin Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit

Product Description

• Columns bind proteins of interest while endotoxins flow through
• Proteins are desalted 
• Reduce endotoxin levels to less than 0.01 EU/µg of protein
• Greater than 95% protein recovery
• Concentrate protein samples and remove detergents at the same time
• Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
• Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix
• Also available as Maxi Kit for up to 4 mg of protein

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is also highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl₂, NaCl, KCl, CaCl₂, LiCl and CsCl.  The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is also designed for the rapid removal of endotoxins from up to 200 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.  Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli.  Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications.  This kit efficiently reduces endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix.  The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes.

Performance Data

Figure 1. Endotoxins were removed from 100 ug protein samples in triplicate using the ProteoSpin ™ Endotoxin Removal Micro Kit. On average, the endotoxin levels in the 100 ug samples were reduced from 0.9 EU/ ug to 0.005 EU/ ug.

Figure 1. Average protein recoveries of greater then 95% were achieved when endotoxins were removed from 100 ug inputs of protein in triplicate.